After drought treatment, marked increase in quantity of PEPcarboxylase protein in the leaves of M. cordifolium, an inducible CAM plant, was shown by SDS-PAGE and western immunoblot (Fig. 1). Further investigation with...After drought treatment, marked increase in quantity of PEPcarboxylase protein in the leaves of M. cordifolium, an inducible CAM plant, was shown by SDS-PAGE and western immunoblot (Fig. 1). Further investigation with incorporation of L-[^(35)S] methionine into leaf discs and immunoprecipitation revealed that de novo synthesis of PEP-carboxylase protein was responsible for this increase (Fig. 2).展开更多
文摘After drought treatment, marked increase in quantity of PEPcarboxylase protein in the leaves of M. cordifolium, an inducible CAM plant, was shown by SDS-PAGE and western immunoblot (Fig. 1). Further investigation with incorporation of L-[^(35)S] methionine into leaf discs and immunoprecipitation revealed that de novo synthesis of PEP-carboxylase protein was responsible for this increase (Fig. 2).