藏麻黄(Ephedra saxatilis Royle ex Florin)是西藏地区特有种,是重要的藏药资源,具有发散风寒、发汗平喘和抗肿瘤等功效。植物相关微生物会影响植物生长发育,并且部分内生真菌影响药用植物有效活性成分的合成。通过比较荒漠草原环境(G...藏麻黄(Ephedra saxatilis Royle ex Florin)是西藏地区特有种,是重要的藏药资源,具有发散风寒、发汗平喘和抗肿瘤等功效。植物相关微生物会影响植物生长发育,并且部分内生真菌影响药用植物有效活性成分的合成。通过比较荒漠草原环境(GHS)和高寒草原环境(GHC)生长的藏麻黄植物内生真菌及根际土壤真菌的群落结构及多样性,挖掘生境差异的真菌组成,以揭示藏麻黄植物的适应性机制,以及挖掘药用真菌资源。通过可培养技术,分离和纯化不同环境藏麻黄根际土壤真菌和根、茎组织内生真菌,并利用ITS区域序列进行分析鉴定。最终得到根际土壤真菌49株,鉴定为15属24种,获得内生真菌54株,鉴定为19属25种。在荒漠草原环境和高寒草原环境生长的藏麻黄根际土壤真菌分别注释到8属13种和7属12种,植物内生真菌分别注释到9属12种和14属16种。多样性分析结果表明,不同环境下菌群的alpha多样性无显著差异。藏麻黄及其根际土壤具有丰富的可培养真菌资源,不同环境的真菌及内生真菌群落结构差异较大。分离、纯化的菌株为进一步开发利用功能菌株资源提供材料。展开更多
This paper describes the quantitative determination of rare-earth elements cerium, dysprosium and non-rare-earth element copper, lead and the homogeneity examination of these four elements in yttrium oxide reference m...This paper describes the quantitative determination of rare-earth elements cerium, dysprosium and non-rare-earth element copper, lead and the homogeneity examination of these four elements in yttrium oxide reference material by isotope dilution spark source mass spectrometry (ID-SSMS). The sensitivity of the meth- od is l0^(-5)～10^(-7%). The precision is better than 6%. The accuracy is better than 5%. The interferences of the spectra and the optimum amount of the spike added to the sample are discussed. The choice of the conducting material for the sample electrodes is studied.展开更多
文摘藏麻黄(Ephedra saxatilis Royle ex Florin)是西藏地区特有种,是重要的藏药资源,具有发散风寒、发汗平喘和抗肿瘤等功效。植物相关微生物会影响植物生长发育,并且部分内生真菌影响药用植物有效活性成分的合成。通过比较荒漠草原环境(GHS)和高寒草原环境(GHC)生长的藏麻黄植物内生真菌及根际土壤真菌的群落结构及多样性,挖掘生境差异的真菌组成,以揭示藏麻黄植物的适应性机制,以及挖掘药用真菌资源。通过可培养技术,分离和纯化不同环境藏麻黄根际土壤真菌和根、茎组织内生真菌,并利用ITS区域序列进行分析鉴定。最终得到根际土壤真菌49株,鉴定为15属24种,获得内生真菌54株,鉴定为19属25种。在荒漠草原环境和高寒草原环境生长的藏麻黄根际土壤真菌分别注释到8属13种和7属12种,植物内生真菌分别注释到9属12种和14属16种。多样性分析结果表明,不同环境下菌群的alpha多样性无显著差异。藏麻黄及其根际土壤具有丰富的可培养真菌资源,不同环境的真菌及内生真菌群落结构差异较大。分离、纯化的菌株为进一步开发利用功能菌株资源提供材料。
文摘This paper describes the quantitative determination of rare-earth elements cerium, dysprosium and non-rare-earth element copper, lead and the homogeneity examination of these four elements in yttrium oxide reference material by isotope dilution spark source mass spectrometry (ID-SSMS). The sensitivity of the meth- od is l0^(-5)～10^(-7%). The precision is better than 6%. The accuracy is better than 5%. The interferences of the spectra and the optimum amount of the spike added to the sample are discussed. The choice of the conducting material for the sample electrodes is studied.
