目的检验小鼠休眠胚胎冻融后的质量及体内外发育潜力,为胚胎休眠技术的生产应用提供必要的参考。方法采用常规冷冻方法将正常孵化期胚胎和休眠胚胎进行冷冻,之后分别进行体外复苏培养实验和胚胎移植实验。随后利用双重荧光染色的方法分...目的检验小鼠休眠胚胎冻融后的质量及体内外发育潜力,为胚胎休眠技术的生产应用提供必要的参考。方法采用常规冷冻方法将正常孵化期胚胎和休眠胚胎进行冷冻,之后分别进行体外复苏培养实验和胚胎移植实验。随后利用双重荧光染色的方法分别对冻融前后的小鼠休眠胚胎与正常孵化期胚胎进行细胞计数,观察两种胚胎冻融前后的质量变化。结果休眠胚胎的冷冻解冻回收率、发育率均极显著高于孵化期胚胎(72.1%vs 50.2%,P<0.01;94.2%vs 73.9%,P<0.01)。休眠胚胎的移植妊娠率显著高于孵化期胚胎(40.8%vs 30.1%,P<0.05)。休眠胚胎的内细胞团细胞数显著高于孵化期胚胎(27.83 vs 19.53,P<0.05),滋养层细胞数差异不显著。冻融培养后休眠胚胎的内细胞团数,滋养层细胞数均显著高于孵化期胚胎(25.18 vs 14.68,P<0.05;114.09 vs 73.88,P<0.05)。结论小鼠休眠胚胎冻融后胚胎质量及体内外发育潜力均优于小鼠正常孵化期胚胎。展开更多
Background Whilst aging is well recognized to be the most significant risk factor for the development of atrial fibrillation(AF),AF also affects young person in whom it is frequently symptomatic and troublesome.The re...Background Whilst aging is well recognized to be the most significant risk factor for the development of atrial fibrillation(AF),AF also affects young person in whom it is frequently symptomatic and troublesome.The relationship between age and prevalence of AF among patients with hypertension remains uncertain.Methods A retrospective cross-sectional study was conducted in consecutively enrolled hypertensive patients aging more than 18 years old from January 1,2013,to December 31,2013,at the Guangdong community in China. AF was systematically screened and diagnosed by rest 12-lead electrocardiogram(ECG)or self-reported. Univariate and multivariate logistic regression,two-piecewise linear regression model and generalized additive model were performed to evaluate the relationship between age and AF. Results There were 7808 participants including 3678(47.1%)men and 4130(52.9%)women(mean age of 62.3 years old)and 78 cases of AF were identified,including 42 men(53.8%). Both univariate and multivariate logistic regression illustrated that age was positively correlated with AF whether age was analyzed as a continuous(all P <0.0001)or categorical(P for trend <0.001)variable. A non-linear correlation was detected after adjusting for potential confounders and the inflection points were 40 and 60 years. Conclusions The relationship between age and AF is non-linear. Age < 40 years is a protective factor for AF,while age > 60 years is significantly correlated with a higher risk of AF.展开更多
Background Little is known regarding the association of changes in blood pressure level with risk of allcause and cardiovascular disease(CVD) mortality in young adults. Methods This cohort study from the 1999-2006 Nat...Background Little is known regarding the association of changes in blood pressure level with risk of allcause and cardiovascular disease(CVD) mortality in young adults. Methods This cohort study from the 1999-2006 National Health and Nutrition Examination Survey(NHANES) consisted of 9977 adults aged from 18 to 40 years by following up until the date of death or December 31, 2015. Participants were categorized by blood pressure readings using the blood pressure classification of the 2017 American College of Cardiology/American Heart Association(ACC/AHA) High Blood Pressure Clinical Practice Guidelines: normal(systolic, <120 mm Hg;diastolic, <80 mm Hg), elevated(systolic, 120-129 mm Hg;diastolic, <80 mm Hg), and hypertension(systolic,≥130 mm Hg;diastolic,≥80 mm Hg). Multivariable Cox proportional hazard models yielded adjusted hazard ratios(HRs) and 95% confidence intervals(CIs) of CVD and all-cause mortality. Results A total of 8356 participants(median age, 26.63 ± 7.01, 3758 women [44.97%]), of whom 265(3.17%) all-cause and 10(0.12%)CVD mortality were observed during a median follow-up duration of 152.96 ± 30.45 months. All-cause mortality incidence rates for normal blood pressure, elevated blood pressure, and hypertension were 172(2.91%), 43(3.52%), and 50(4.10%), respectively. With the normal blood pressure group being a reference, from elevated blood pressure to hypertension group, adjusted HRs for all-cause mortality were 1.24(95% CI, 0.63-2.42) and1.52(95% CI, 0.83-2.80)(P=0.162) after adjustment for potential confounders. Conclusions Among young adults, those with elevated blood pressure and hypertension, compared with those with normal blood pressure before the age of 40, as defined by the blood pressure classification in the 2017 ACC/AHA guidelines, are not significantly associated with increased risk of subsequent all-cause mortality.[S Chin J Cardiol 2019;20(4):201-210]展开更多
文摘目的检验小鼠休眠胚胎冻融后的质量及体内外发育潜力,为胚胎休眠技术的生产应用提供必要的参考。方法采用常规冷冻方法将正常孵化期胚胎和休眠胚胎进行冷冻,之后分别进行体外复苏培养实验和胚胎移植实验。随后利用双重荧光染色的方法分别对冻融前后的小鼠休眠胚胎与正常孵化期胚胎进行细胞计数,观察两种胚胎冻融前后的质量变化。结果休眠胚胎的冷冻解冻回收率、发育率均极显著高于孵化期胚胎(72.1%vs 50.2%,P<0.01;94.2%vs 73.9%,P<0.01)。休眠胚胎的移植妊娠率显著高于孵化期胚胎(40.8%vs 30.1%,P<0.05)。休眠胚胎的内细胞团细胞数显著高于孵化期胚胎(27.83 vs 19.53,P<0.05),滋养层细胞数差异不显著。冻融培养后休眠胚胎的内细胞团数,滋养层细胞数均显著高于孵化期胚胎(25.18 vs 14.68,P<0.05;114.09 vs 73.88,P<0.05)。结论小鼠休眠胚胎冻融后胚胎质量及体内外发育潜力均优于小鼠正常孵化期胚胎。
基金supported by the Science and Technology Program of Guangzhou(No.201604020143,No.201604020018,No.201604020186,and No.201803040012)the National Key Research and Development Program of China(No.2017YFC1307603/No.2016YFC1301305)the Key Area R&D Program of Guangdong Province(No.2019B020227005)
文摘Background Whilst aging is well recognized to be the most significant risk factor for the development of atrial fibrillation(AF),AF also affects young person in whom it is frequently symptomatic and troublesome.The relationship between age and prevalence of AF among patients with hypertension remains uncertain.Methods A retrospective cross-sectional study was conducted in consecutively enrolled hypertensive patients aging more than 18 years old from January 1,2013,to December 31,2013,at the Guangdong community in China. AF was systematically screened and diagnosed by rest 12-lead electrocardiogram(ECG)or self-reported. Univariate and multivariate logistic regression,two-piecewise linear regression model and generalized additive model were performed to evaluate the relationship between age and AF. Results There were 7808 participants including 3678(47.1%)men and 4130(52.9%)women(mean age of 62.3 years old)and 78 cases of AF were identified,including 42 men(53.8%). Both univariate and multivariate logistic regression illustrated that age was positively correlated with AF whether age was analyzed as a continuous(all P <0.0001)or categorical(P for trend <0.001)variable. A non-linear correlation was detected after adjusting for potential confounders and the inflection points were 40 and 60 years. Conclusions The relationship between age and AF is non-linear. Age < 40 years is a protective factor for AF,while age > 60 years is significantly correlated with a higher risk of AF.
基金supported by the Science and Technology Program of Guangzhou(No.201604020143/No.201604020018/No.201604020186/No.201803040012)the National Key Research and Development Program of China(No.2017FYC1307603/No.2016YFC1301305)the Key Area R&D Program of Guangdong Province(No.2019B020227005)
文摘Background Little is known regarding the association of changes in blood pressure level with risk of allcause and cardiovascular disease(CVD) mortality in young adults. Methods This cohort study from the 1999-2006 National Health and Nutrition Examination Survey(NHANES) consisted of 9977 adults aged from 18 to 40 years by following up until the date of death or December 31, 2015. Participants were categorized by blood pressure readings using the blood pressure classification of the 2017 American College of Cardiology/American Heart Association(ACC/AHA) High Blood Pressure Clinical Practice Guidelines: normal(systolic, <120 mm Hg;diastolic, <80 mm Hg), elevated(systolic, 120-129 mm Hg;diastolic, <80 mm Hg), and hypertension(systolic,≥130 mm Hg;diastolic,≥80 mm Hg). Multivariable Cox proportional hazard models yielded adjusted hazard ratios(HRs) and 95% confidence intervals(CIs) of CVD and all-cause mortality. Results A total of 8356 participants(median age, 26.63 ± 7.01, 3758 women [44.97%]), of whom 265(3.17%) all-cause and 10(0.12%)CVD mortality were observed during a median follow-up duration of 152.96 ± 30.45 months. All-cause mortality incidence rates for normal blood pressure, elevated blood pressure, and hypertension were 172(2.91%), 43(3.52%), and 50(4.10%), respectively. With the normal blood pressure group being a reference, from elevated blood pressure to hypertension group, adjusted HRs for all-cause mortality were 1.24(95% CI, 0.63-2.42) and1.52(95% CI, 0.83-2.80)(P=0.162) after adjustment for potential confounders. Conclusions Among young adults, those with elevated blood pressure and hypertension, compared with those with normal blood pressure before the age of 40, as defined by the blood pressure classification in the 2017 ACC/AHA guidelines, are not significantly associated with increased risk of subsequent all-cause mortality.[S Chin J Cardiol 2019;20(4):201-210]
