To study the internalization of immunotargeting drugs for hepatocellular carcinoma. Methods: By using colloidal gold technique, the processes of internalization of the immunotargeting drugs, HAb18 and HAb25, against h...To study the internalization of immunotargeting drugs for hepatocellular carcinoma. Methods: By using colloidal gold technique, the processes of internalization of the immunotargeting drugs, HAb18 and HAb25, against hepatoma in the targeting cells of human hepatoma cell line SMMC-7721 were observed separatelly ctively under an electron microscope. Results: 80% of the target cells were conjugated with gold-labeled particles on the cellular surfaces. The internalization of gold-labeled particles were present in all of the target cells conjugated with gold-labeled particles, while no internalization of gold-labeled particles could be seen in the target cells not conjugated with gold-labeled particles. The chief way of internalization was invariably through a non-coated microinvagination. After entering the cells. all of the gold-labled particles were first localized in the primary endocytic vacuoles, and then transferred intracellularly. Simultaneously, the vacuole-vacuole fusion occurred forming the larger multi-vesicular bodies, the endosome. In the Golgi region, the endosome fused with the Golgic vacuoles, and finally located in the secondary lysosomes. 18h after the intercellular internalization of the immunotargeting drugs, the cytoplasmic vacuolization, and sometimes even cellular necrosis, could be noticed. The control cells grew well. Conclusion: After entering the targeting cells, the immunotargeting drugs would degrade within the lysosomes. And when ADM arrives at its function site it would play the role of cell toxicity intranuclearly.Therefore, internalization of HAb18-ADM and HAb25-ADM might have a good prospect in clinical application.展开更多
文摘To study the internalization of immunotargeting drugs for hepatocellular carcinoma. Methods: By using colloidal gold technique, the processes of internalization of the immunotargeting drugs, HAb18 and HAb25, against hepatoma in the targeting cells of human hepatoma cell line SMMC-7721 were observed separatelly ctively under an electron microscope. Results: 80% of the target cells were conjugated with gold-labeled particles on the cellular surfaces. The internalization of gold-labeled particles were present in all of the target cells conjugated with gold-labeled particles, while no internalization of gold-labeled particles could be seen in the target cells not conjugated with gold-labeled particles. The chief way of internalization was invariably through a non-coated microinvagination. After entering the cells. all of the gold-labled particles were first localized in the primary endocytic vacuoles, and then transferred intracellularly. Simultaneously, the vacuole-vacuole fusion occurred forming the larger multi-vesicular bodies, the endosome. In the Golgi region, the endosome fused with the Golgic vacuoles, and finally located in the secondary lysosomes. 18h after the intercellular internalization of the immunotargeting drugs, the cytoplasmic vacuolization, and sometimes even cellular necrosis, could be noticed. The control cells grew well. Conclusion: After entering the targeting cells, the immunotargeting drugs would degrade within the lysosomes. And when ADM arrives at its function site it would play the role of cell toxicity intranuclearly.Therefore, internalization of HAb18-ADM and HAb25-ADM might have a good prospect in clinical application.
