本文以牛膀胱变移上皮为材料,利用石蜡切片、免疫荧光及多种电镜技术,对其形态结构进行了较为系统的研究,并对上皮细胞膜蛋白 Uroplakin I 的定位做了初步的探讨。在显微与亚显微水平上,牛膀胱变移上皮细胞大致可分为三类,即富含膜泡的...本文以牛膀胱变移上皮为材料,利用石蜡切片、免疫荧光及多种电镜技术,对其形态结构进行了较为系统的研究,并对上皮细胞膜蛋白 Uroplakin I 的定位做了初步的探讨。在显微与亚显微水平上,牛膀胱变移上皮细胞大致可分为三类,即富含膜泡的表层细胞,富含线粒体的中间层细胞和其下的基底层细胞。在超薄切片样品中其腔面细胞质膜由扇形凹陷区与冠状突起区相间排列而成,细胞质膜的暗一亮一暗单位膜结构可以清晰地分辨出来,靠腔面厚约80A,靠细胞质面厚约40A,表现出明显的不对称性(图1),因而称之为不对称单位膜。冰冻断裂复型样品中在细胞质膜纵断面上,表层细胞腔面膜的形状与超薄切片中类似,亦由扇形凹陷区与冠状突起区相间排列而成。扇形凹陷区即为斑区,分布着许多的颗粒状结构。展开更多
Ⅰ. INTRODUCTIONNuclear reconstitution (nuclear assembly )was reported by several laboratories in recent years and regarded as an important advance in cell biology. In vitro nuclear assembly system provides a good mod...Ⅰ. INTRODUCTIONNuclear reconstitution (nuclear assembly )was reported by several laboratories in recent years and regarded as an important advance in cell biology. In vitro nuclear assembly system provides a good model to study the organization and function of nucleus, chromatin, nuclear envelope and nuclear matrix. Although it is too early to evaluate its significance in theory and in bioengineering, there is no doubt that the prospect in this field is inspiring.展开更多
文摘本文以牛膀胱变移上皮为材料,利用石蜡切片、免疫荧光及多种电镜技术,对其形态结构进行了较为系统的研究,并对上皮细胞膜蛋白 Uroplakin I 的定位做了初步的探讨。在显微与亚显微水平上,牛膀胱变移上皮细胞大致可分为三类,即富含膜泡的表层细胞,富含线粒体的中间层细胞和其下的基底层细胞。在超薄切片样品中其腔面细胞质膜由扇形凹陷区与冠状突起区相间排列而成,细胞质膜的暗一亮一暗单位膜结构可以清晰地分辨出来,靠腔面厚约80A,靠细胞质面厚约40A,表现出明显的不对称性(图1),因而称之为不对称单位膜。冰冻断裂复型样品中在细胞质膜纵断面上,表层细胞腔面膜的形状与超薄切片中类似,亦由扇形凹陷区与冠状突起区相间排列而成。扇形凹陷区即为斑区,分布着许多的颗粒状结构。
基金This work was supported by grants from the National Natral Science Foundation of China and Doctoral Fondstion from the National Committee for Education.
文摘Ⅰ. INTRODUCTIONNuclear reconstitution (nuclear assembly )was reported by several laboratories in recent years and regarded as an important advance in cell biology. In vitro nuclear assembly system provides a good model to study the organization and function of nucleus, chromatin, nuclear envelope and nuclear matrix. Although it is too early to evaluate its significance in theory and in bioengineering, there is no doubt that the prospect in this field is inspiring.
