以碱性磷酸酶(ALP)、镁激活的腺苷三磷酸酶(Mg^(2+)-ALP_(ase))和儿茶酚胺(CA)荧光组化方法观察了电针对急性心肌缺血作用的机理。结扎左冠状动脉心室支30分钟后,缺血心肌中以 ATP 显示的毛细血管大为减少,长度缩短;而缺血电针组则有显...以碱性磷酸酶(ALP)、镁激活的腺苷三磷酸酶(Mg^(2+)-ALP_(ase))和儿茶酚胺(CA)荧光组化方法观察了电针对急性心肌缺血作用的机理。结扎左冠状动脉心室支30分钟后,缺血心肌中以 ATP 显示的毛细血管大为减少,长度缩短;而缺血电针组则有显著意义增加,说明电针对缺血心肌微循环和转运功能的改善。Mg^(2+)-ATP_(ase)可显示心肌微血管的质膜和基膜;经显微分光光度计测量,Mg^(2+)-ATP_(ase)在缺血电针组比缺血对照组光密度增加,这可加强缺血心肌 Na^+K^+离子跨膜转动功能。心肌儿茶酚胺荧光所显示的交感肾上腺素能膨体神经末梢;缺血后减少或耗竭,电针组则较多而亮,这有益于加强缺血心肌的收缩力。展开更多
Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units...Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units in AMYG caused by AMI,that is,EA inhibited theincreased frequency,and reversed the decreased frequency.This suggested that AMYG is an im-portant link in correlation between the heart and the acupoint Neiguan,providing an experimen-tal basis for the Theory of Channel-Viscera Correlation.展开更多
In this paper,the regularity on spontaneous dis-charges of the single unit in amygdaloid nucleus(AMYG)in rabbits were analyzed and whether thesignal of Electro-acupuncture(EA)at Neignan (P 6)can reach the AMYG was fur...In this paper,the regularity on spontaneous dis-charges of the single unit in amygdaloid nucleus(AMYG)in rabbits were analyzed and whether thesignal of Electro-acupuncture(EA)at Neignan (P 6)can reach the AMYG was further observed withthe technique of glass microelectrode.It was foundthat the signals of EA at Neignan can reach AMYGand activate or inhibit the electrical activity of somencurons,manifesting two response patterns:fre-quency-increasing and frequency-decreasing.Addi-tionally,the same neuron had a different response tothe signals of EA at Neiguan and Zusanli(St 36),suggesting that a relative specificity of acupoints ispresent.展开更多
In the present paper, the effect of α1 and α2 subtypes of the thoracic spinal cord on electroacupuncture (EA)-induced improvement of ischemic cardiac electrical and mechanical activities was observed in 80 anestheti...In the present paper, the effect of α1 and α2 subtypes of the thoracic spinal cord on electroacupuncture (EA)-induced improvement of ischemic cardiac electrical and mechanical activities was observed in 80 anesthetized rabbits by using ECG-ST, MBP, LVP and dp/dt max as indexes. Results showed that:1) EA at "Neiguan" could significantly improve the electrical and mechanical activities of the ischemic heart; 2) the effect of EA could be enhanced to a certain degree when α1 receptors of the thoracic spinal cord were activated by subarachnoid microinjection of phenylephrine, while it was weakened when α1 receptors were inhibited by microinjection of parison; and 3) activation or inhibition of α2 receptors of the thoracic spinal cord by microinjection of clonidine and yohimbine had no marked influence on the effect of EA in improving electrical activity of the ischemic heart, but when activated,they could weaken the effect of EA in raising MBP; while suppressed, they had no any striking influence on the effect of EA in raising MBP. It suggests that among α-receptors, predominantly α1-receptors participate in the process of EA-induced improvement of performance of ischemic heart.展开更多
文摘以碱性磷酸酶(ALP)、镁激活的腺苷三磷酸酶(Mg^(2+)-ALP_(ase))和儿茶酚胺(CA)荧光组化方法观察了电针对急性心肌缺血作用的机理。结扎左冠状动脉心室支30分钟后,缺血心肌中以 ATP 显示的毛细血管大为减少,长度缩短;而缺血电针组则有显著意义增加,说明电针对缺血心肌微循环和转运功能的改善。Mg^(2+)-ATP_(ase)可显示心肌微血管的质膜和基膜;经显微分光光度计测量,Mg^(2+)-ATP_(ase)在缺血电针组比缺血对照组光密度增加,这可加强缺血心肌 Na^+K^+离子跨膜转动功能。心肌儿茶酚胺荧光所显示的交感肾上腺素能膨体神经末梢;缺血后减少或耗竭,电针组则较多而亮,这有益于加强缺血心肌的收缩力。
基金The project was supported by National Natural Science Foundation of China.
文摘Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units in AMYG caused by AMI,that is,EA inhibited theincreased frequency,and reversed the decreased frequency.This suggested that AMYG is an im-portant link in correlation between the heart and the acupoint Neiguan,providing an experimen-tal basis for the Theory of Channel-Viscera Correlation.
文摘In this paper,the regularity on spontaneous dis-charges of the single unit in amygdaloid nucleus(AMYG)in rabbits were analyzed and whether thesignal of Electro-acupuncture(EA)at Neignan (P 6)can reach the AMYG was further observed withthe technique of glass microelectrode.It was foundthat the signals of EA at Neignan can reach AMYGand activate or inhibit the electrical activity of somencurons,manifesting two response patterns:fre-quency-increasing and frequency-decreasing.Addi-tionally,the same neuron had a different response tothe signals of EA at Neiguan and Zusanli(St 36),suggesting that a relative specificity of acupoints ispresent.
文摘In the present paper, the effect of α1 and α2 subtypes of the thoracic spinal cord on electroacupuncture (EA)-induced improvement of ischemic cardiac electrical and mechanical activities was observed in 80 anesthetized rabbits by using ECG-ST, MBP, LVP and dp/dt max as indexes. Results showed that:1) EA at "Neiguan" could significantly improve the electrical and mechanical activities of the ischemic heart; 2) the effect of EA could be enhanced to a certain degree when α1 receptors of the thoracic spinal cord were activated by subarachnoid microinjection of phenylephrine, while it was weakened when α1 receptors were inhibited by microinjection of parison; and 3) activation or inhibition of α2 receptors of the thoracic spinal cord by microinjection of clonidine and yohimbine had no marked influence on the effect of EA in improving electrical activity of the ischemic heart, but when activated,they could weaken the effect of EA in raising MBP; while suppressed, they had no any striking influence on the effect of EA in raising MBP. It suggests that among α-receptors, predominantly α1-receptors participate in the process of EA-induced improvement of performance of ischemic heart.
