It was found from experiments that the addition of polythylene glycol (PEG) can make the B-Z and B-R reactions producing double induction periods, i.e. before the general induction period there is an earlier stage of ...It was found from experiments that the addition of polythylene glycol (PEG) can make the B-Z and B-R reactions producing double induction periods, i.e. before the general induction period there is an earlier stage of induction in which there is no any change of color and state. The lifetime of the earlier stage is not noly effected by the concentration of PEG, but also by the time of the last reagent added. It was also found that there are two linear relationships between the concentration of PEG and the reduced viscodity of PEG solution or/and the lifetime of the earlier stage of induction. Another characteristic of PEG effect is that it can decrease the period change during the oscillat ing reactions. These experimental results are not only useful in the research pf chemical oscillation, but also give a simple method for determining colligative properties of macro molecule sultion.展开更多
The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse Ancelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’-p...The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse Ancelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’-phosphate as the substrate, and it was found that kcat increases significantly with respect to that in water attended by an increased Km·FT-IR spectra of RNaseA in reverse Ancellax solution were investigated as a function of w0(= [H2O]/ [DAB]), and it was noted that the structure of RNaseA became looser in reverse micelles campared to that in aqueous solution. The relation between activity and conformation was discussed.展开更多
The enzymetic activity of RNaseA solubilized in reverse micelles formed in cyclohexane by dodecylammonium butyrate(DAB) and water has been investigated with the use of cytidine 2’, 3’-phosphate as the substrate. In ...The enzymetic activity of RNaseA solubilized in reverse micelles formed in cyclohexane by dodecylammonium butyrate(DAB) and water has been investigated with the use of cytidine 2’, 3’-phosphate as the substrate. In the investigated concentration range, RNaseA exhibited a "superactivity" in the DAB reverse micelle.展开更多
文摘It was found from experiments that the addition of polythylene glycol (PEG) can make the B-Z and B-R reactions producing double induction periods, i.e. before the general induction period there is an earlier stage of induction in which there is no any change of color and state. The lifetime of the earlier stage is not noly effected by the concentration of PEG, but also by the time of the last reagent added. It was also found that there are two linear relationships between the concentration of PEG and the reduced viscodity of PEG solution or/and the lifetime of the earlier stage of induction. Another characteristic of PEG effect is that it can decrease the period change during the oscillat ing reactions. These experimental results are not only useful in the research pf chemical oscillation, but also give a simple method for determining colligative properties of macro molecule sultion.
文摘The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse Ancelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’-phosphate as the substrate, and it was found that kcat increases significantly with respect to that in water attended by an increased Km·FT-IR spectra of RNaseA in reverse Ancellax solution were investigated as a function of w0(= [H2O]/ [DAB]), and it was noted that the structure of RNaseA became looser in reverse micelles campared to that in aqueous solution. The relation between activity and conformation was discussed.
文摘The enzymetic activity of RNaseA solubilized in reverse micelles formed in cyclohexane by dodecylammonium butyrate(DAB) and water has been investigated with the use of cytidine 2’, 3’-phosphate as the substrate. In the investigated concentration range, RNaseA exhibited a "superactivity" in the DAB reverse micelle.
