To synthesize three different grafting ratios of gallic acid(GA)-chitosan(CS)copolymer by a free radical mediated grafting method,which is further applied to the field of antibacterial materials,crosslinking structure...To synthesize three different grafting ratios of gallic acid(GA)-chitosan(CS)copolymer by a free radical mediated grafting method,which is further applied to the field of antibacterial materials,crosslinking structures of the compound GA-CS copolymer were characterized,fully indicating that gallic acid is resoundingly grafted onto chitosan.The grafting ratios of three copolymers GA-CS are 45.71%(Ⅰ),36.12%(Ⅱ),and 18.96%(Ⅲ)were determined by UV-Vis spectrophotometer.The minimum inhibitory concentrations of three GA-CS copolymers are 30μg/mL against Escherichia coli and are ranged from 250 to 550μg/mL against Staphylococcus aureus.By counting viable bacterial colonies,it can be found that antibacterial property is preferable by increasing the grafting ratio of GA-CS copolymers.Findings of investigation on aforementioned bacteria experiment indicate that the CFU/mL values of GA-CS(Ⅰ,Ⅱ,Ⅲ)are 2.04×10^(6),7.56×10^(6),1.48×10^(7) to Staphylococcus aureus,and 2.96×10^(6),1.01×10^(7),2.14×10^(7) to Escherichia coli after 12 h treatment.In addition,the interaction process between GA-CS copolymer and bacteria can be observed through a transmission electron microscope.The specific manifestation is that the bacterial cell membranes are ruptured after being treated with the copolymer,which causes the cell contents to flow out,and the cell morphology is shrunk and out ofshape.展开更多
目的:通过建立高脂血症大鼠模型对富含膳食纤维和不饱和脂肪酸的复方海带代餐粉进行辅助降血脂作用研究。方法:采用SD雄性大鼠,设空白对照组、模型对照组和复方海带粉高、中、低3个剂量组:6.58,3.29,1.65 g/kg(分别相当于人体推荐量的10...目的:通过建立高脂血症大鼠模型对富含膳食纤维和不饱和脂肪酸的复方海带代餐粉进行辅助降血脂作用研究。方法:采用SD雄性大鼠,设空白对照组、模型对照组和复方海带粉高、中、低3个剂量组:6.58,3.29,1.65 g/kg(分别相当于人体推荐量的10,5,2.5倍),空白对照组饲喂基础饲料,其他组饲喂高脂饲料,3个剂量组灌胃给予相应浓度的复方海带粉混悬液,4周后,测定血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low density lipoprotein,LDL-C)和高密度脂蛋白胆固醇(High density lipoprotein,HDL-C)水平,并计算动脉粥样硬化指数(Atherosclerosis index,AI)。结果:3组剂量的复方海带粉均能降低大鼠血清TC(低剂量组P<0.05,中、高剂量组P<0.01)、TG(P<0.05)和LDL-C水平(低剂量组P<0.05,中、高剂量组P<0.01),提高HDL-C水平(中、高剂量组P<0.01),且存在一定的剂量依赖关系;同时,还能有效抑制高脂大鼠动脉粥样硬化指数的升高(P<0.01)。结论:复方海带代餐粉具有较好的辅助降血脂及抗动脉粥样硬化作用。展开更多
基金Funded by the Health Commission of Hubei Province Scientific Research Project(No.WJ2019H275)。
文摘To synthesize three different grafting ratios of gallic acid(GA)-chitosan(CS)copolymer by a free radical mediated grafting method,which is further applied to the field of antibacterial materials,crosslinking structures of the compound GA-CS copolymer were characterized,fully indicating that gallic acid is resoundingly grafted onto chitosan.The grafting ratios of three copolymers GA-CS are 45.71%(Ⅰ),36.12%(Ⅱ),and 18.96%(Ⅲ)were determined by UV-Vis spectrophotometer.The minimum inhibitory concentrations of three GA-CS copolymers are 30μg/mL against Escherichia coli and are ranged from 250 to 550μg/mL against Staphylococcus aureus.By counting viable bacterial colonies,it can be found that antibacterial property is preferable by increasing the grafting ratio of GA-CS copolymers.Findings of investigation on aforementioned bacteria experiment indicate that the CFU/mL values of GA-CS(Ⅰ,Ⅱ,Ⅲ)are 2.04×10^(6),7.56×10^(6),1.48×10^(7) to Staphylococcus aureus,and 2.96×10^(6),1.01×10^(7),2.14×10^(7) to Escherichia coli after 12 h treatment.In addition,the interaction process between GA-CS copolymer and bacteria can be observed through a transmission electron microscope.The specific manifestation is that the bacterial cell membranes are ruptured after being treated with the copolymer,which causes the cell contents to flow out,and the cell morphology is shrunk and out ofshape.
文摘目的:通过建立高脂血症大鼠模型对富含膳食纤维和不饱和脂肪酸的复方海带代餐粉进行辅助降血脂作用研究。方法:采用SD雄性大鼠,设空白对照组、模型对照组和复方海带粉高、中、低3个剂量组:6.58,3.29,1.65 g/kg(分别相当于人体推荐量的10,5,2.5倍),空白对照组饲喂基础饲料,其他组饲喂高脂饲料,3个剂量组灌胃给予相应浓度的复方海带粉混悬液,4周后,测定血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low density lipoprotein,LDL-C)和高密度脂蛋白胆固醇(High density lipoprotein,HDL-C)水平,并计算动脉粥样硬化指数(Atherosclerosis index,AI)。结果:3组剂量的复方海带粉均能降低大鼠血清TC(低剂量组P<0.05,中、高剂量组P<0.01)、TG(P<0.05)和LDL-C水平(低剂量组P<0.05,中、高剂量组P<0.01),提高HDL-C水平(中、高剂量组P<0.01),且存在一定的剂量依赖关系;同时,还能有效抑制高脂大鼠动脉粥样硬化指数的升高(P<0.01)。结论:复方海带代餐粉具有较好的辅助降血脂及抗动脉粥样硬化作用。
