自适应光学在超分辨荧光显微镜中的应用

超分辨荧光显微镜突破了传统荧光显微镜的分辨率限制,使得人们能够在纳米量级分辨率下观察细胞和组织样品,极大地推动了生命科学的发展。在这一技术中,仪器和样品引入的像差均会导致空间分辨率降低,进而导致成像质量恶化。为此,人们引入了自适应光学技术,通过直接或间接的手段探测像差,再通过波前校正元件来校正像差,从而获得高质量的超分辨图像。本文介绍了自适应光学的起源与工作原理,总结了其在超分辨荧光显微镜中的应用,并展望了其未来的发展前景。

Reducing the stray light of holographic gratings by shifting the substrate a short distance in the direction parallel or perpendicular to the exposure interference fringes

This research proposes a simple and practical method to make low-stray-light gratings, where the substrate shifts about a 1 mm distance in the direction parallel or perpendicular to the exposure interference fringes. When the substrate shifts, a reference grating next to the substrate is used to adjust in real time the phase of the exposure interference fringes relative to the substrate. Shifting eliminates the exposure defects and therefore decreases the stray light of gratings. Several gratings are successfully made by using this method, which have straighter grooves,smoother surfaces, and lower stray light than gratings made in conventional interference lithography.

和时间做朋友是一种怎样感觉

与时间为友,生活充实而美好自从我怀孕后,难免时间紧张。但我还是想寻找更多生命中的可能,早前些年埋头书籍与学海的时间,积累了心智力量,想着"有些事,现在不做,这辈子可能没机会",另外也想着给自己的下一代做榜样,所以才有动力。当时最先想到的是,在所在的小城市里发起个人管理与成长分享活动——幸福成长沙龙。