目的探讨联合高分辨率食管测压(HREM)及24 h pH监测评估内镜阴性的老年胃灼热患者疾病分布特点和食管运动功能及其临床价值。方法回顾性分析同期行胃镜、HREM和24 h pH监测的老年胃灼热患者91例,分析其食管动力障碍类型及HREM相关参数变...目的探讨联合高分辨率食管测压(HREM)及24 h pH监测评估内镜阴性的老年胃灼热患者疾病分布特点和食管运动功能及其临床价值。方法回顾性分析同期行胃镜、HREM和24 h pH监测的老年胃灼热患者91例,分析其食管动力障碍类型及HREM相关参数变化,并结合24 h pH监测对病因进行诊断和评估。结果基于HREM芝加哥分类标准v4.0,筛选出重度食管动力障碍患者21例(包括5例贲门失弛缓症,4例EGJ流出道梗阻,8例食管无收缩,3例远端食管痉挛,1例高收缩食管)和食管裂孔疝2例。其余68例患者食管测压正常者45例(66.2%),无效食管动力(IEM)23例(33.8%);进一步根据罗马Ⅳ标准结合pH监测诊断分组,非糜烂性反流病(NERD)组24例,反流高敏感(RH)组15例,功能性胃灼热(FH)组29例。各组性别构成、体质量指数(BMI)、食管动力类型和EGJ分型差异均无统计学意义(均P>0.05)。NERD组、RH组和FH组远端收缩积分(DCI)均明显低于正常对照组(P<0.05)。结论内镜阴性的老年胃灼热患者临床诊断异质性大,不能单独依靠内镜及症状诊断,有必要通过HREM排除严重的食管动力障碍性疾病,联合pH监测可以鉴别功能性食管疾病和筛选真正的GERD。IEM是最常见的食管动力障碍类型,但绝大部分患者食管测压是正常的。展开更多
Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we s...Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we synthesized a novel schiff base zinc coordination compound（SBZCC） and investigated its effects on the growth,proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression,mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover,SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis,accompanied with increased Bax/Bcl-2 and Flas L/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways,suggesting that SBZCC is a promising agent for the development as anticancer drugs.展开更多
文摘目的探讨联合高分辨率食管测压(HREM)及24 h pH监测评估内镜阴性的老年胃灼热患者疾病分布特点和食管运动功能及其临床价值。方法回顾性分析同期行胃镜、HREM和24 h pH监测的老年胃灼热患者91例,分析其食管动力障碍类型及HREM相关参数变化,并结合24 h pH监测对病因进行诊断和评估。结果基于HREM芝加哥分类标准v4.0,筛选出重度食管动力障碍患者21例(包括5例贲门失弛缓症,4例EGJ流出道梗阻,8例食管无收缩,3例远端食管痉挛,1例高收缩食管)和食管裂孔疝2例。其余68例患者食管测压正常者45例(66.2%),无效食管动力(IEM)23例(33.8%);进一步根据罗马Ⅳ标准结合pH监测诊断分组,非糜烂性反流病(NERD)组24例,反流高敏感(RH)组15例,功能性胃灼热(FH)组29例。各组性别构成、体质量指数(BMI)、食管动力类型和EGJ分型差异均无统计学意义(均P>0.05)。NERD组、RH组和FH组远端收缩积分(DCI)均明显低于正常对照组(P<0.05)。结论内镜阴性的老年胃灼热患者临床诊断异质性大,不能单独依靠内镜及症状诊断,有必要通过HREM排除严重的食管动力障碍性疾病,联合pH监测可以鉴别功能性食管疾病和筛选真正的GERD。IEM是最常见的食管动力障碍类型,但绝大部分患者食管测压是正常的。
基金supported by grants from the Scientific Research Foundation of Hebei Provincial Health Department,China(No.ZL20140116)the Clinical Research Special Fund of Wu Jieping Medical Foundation(No.320.6750.14119)
文摘Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we synthesized a novel schiff base zinc coordination compound（SBZCC） and investigated its effects on the growth,proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression,mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover,SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis,accompanied with increased Bax/Bcl-2 and Flas L/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways,suggesting that SBZCC is a promising agent for the development as anticancer drugs.
