采用12L玻璃发酵罐,测定了苏芸金杆菌(Bacillus thuringiensis, B. t)发酵过程中的菌体浓度、总糖、还原糖、氨基氮、溶磷、溶氧、铵离子、核酸、ATP、pH、蛋白酶及粘度等参数。获得了B.t生长过程的代谢曲线,从中分析了各参数变化间的...采用12L玻璃发酵罐,测定了苏芸金杆菌(Bacillus thuringiensis, B. t)发酵过程中的菌体浓度、总糖、还原糖、氨基氮、溶磷、溶氧、铵离子、核酸、ATP、pH、蛋白酶及粘度等参数。获得了B.t生长过程的代谢曲线,从中分析了各参数变化间的相互关系。这对于控制B.t发酵过程及提高发酵水平具有一定指导意义。展开更多
It was reported that addition of soybean oil results the variations of on line parameters,such as ECO 2,EO 2,CER,OUR,DO,RQ,K La and so on,during the fermentation and their co relation was analyzed in this paper.It has...It was reported that addition of soybean oil results the variations of on line parameters,such as ECO 2,EO 2,CER,OUR,DO,RQ,K La and so on,during the fermentation and their co relation was analyzed in this paper.It has show that:first,the function of soybean oil were different at different phases of fermentation through parameter co relative analysis,namely soybean oil was an anti foam regent and a carbon resource substrate at the middle phase of fermentation,while the oil was only an anti foam regent at the final phase.Second,the uses of metabolic substrates will be changed when the soybean oil was a carbone resource to some extent that was related with the carbon resource restriction and cell′s activity.Third,the affection factors at balance of oxygen can be realized to optimize the soybean oil feed through parameter co relative analysis.展开更多
文摘采用12L玻璃发酵罐,测定了苏芸金杆菌(Bacillus thuringiensis, B. t)发酵过程中的菌体浓度、总糖、还原糖、氨基氮、溶磷、溶氧、铵离子、核酸、ATP、pH、蛋白酶及粘度等参数。获得了B.t生长过程的代谢曲线,从中分析了各参数变化间的相互关系。这对于控制B.t发酵过程及提高发酵水平具有一定指导意义。
文摘It was reported that addition of soybean oil results the variations of on line parameters,such as ECO 2,EO 2,CER,OUR,DO,RQ,K La and so on,during the fermentation and their co relation was analyzed in this paper.It has show that:first,the function of soybean oil were different at different phases of fermentation through parameter co relative analysis,namely soybean oil was an anti foam regent and a carbon resource substrate at the middle phase of fermentation,while the oil was only an anti foam regent at the final phase.Second,the uses of metabolic substrates will be changed when the soybean oil was a carbone resource to some extent that was related with the carbon resource restriction and cell′s activity.Third,the affection factors at balance of oxygen can be realized to optimize the soybean oil feed through parameter co relative analysis.
