[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups...[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups: control group (drug-free group), 1.5% close group, 1.0% dose group and 0.5% dose group. Blood samples of piglets were collected at 20, 35, 50, 60, 70 and 80 days old, respective- ly, to determine neutrophil leukocyte percentage in peripheral blood, lymphocyte trapsformation rate and the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder. [Result] Applying a certain dose of Echinacea compound could significantly increase neutrophil leukocyte per- centage in peripheral blood and lymphocyte transformation rate (P〈0.05), and ex- tremely significantly improve the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder (P〈0.01). [Conclusion] Echi- nacea compound has played a certain role in promoting nonspecific and specific im- mune function of piglets.展开更多
[目的]旨在研究中兽药复方紫黄散对免疫抑制小鼠细胞因子的影响。[方法]选取100只昆明系小鼠,利用氢化可的松建立免疫抑制小鼠模型。选取造模成功的小鼠60只,随机分为3组,即模型组、紫黄散组和阳性对照组,分别给予0.25 m L/(10 g·B...[目的]旨在研究中兽药复方紫黄散对免疫抑制小鼠细胞因子的影响。[方法]选取100只昆明系小鼠,利用氢化可的松建立免疫抑制小鼠模型。选取造模成功的小鼠60只,随机分为3组,即模型组、紫黄散组和阳性对照组,分别给予0.25 m L/(10 g·BW·d)的生理盐水、紫黄散口服液及玉屏风口服液,均连续灌胃14 d。另取健康小鼠20只,作为健康对照组。测定并比较给药第0天、第7天、第14天各组小鼠的血液WBC含量及血清中细胞因子IL-2、IL-4、TNF-α和IFN-γ含量。[结果]模型组小鼠给药第0天的血液WBC含量显著低于健康对照组(P<0.05),表明免疫抑制小鼠模型造模成功;给药第7天和第14天,紫黄散组小鼠的血液WBC含量显著高于模型组(P<0.05),且紫黄散组小鼠给药第7天和第14天的血液WBC含量显著高于给药第0天(P<0.05);给药第7天及第14天,紫黄散组小鼠血清IL-2、IL-4、TNF-α和IFN-γ含量显著高于模型组(P<0.05),且与健康对照组及阳性对照组差异不显著(P>0.05)。[结论]紫黄散口服液能够显著提高免疫抑制小鼠的细胞免疫和体液免疫水平,具有一定的免疫增强作用。展开更多
基金Supported by National Nature Science Foundation of China(31472230)Nature Science Foundation of Hebei Province(C2014407068)+2 种基金Project of Science and Technology Department of Hebei Province(14966610D)Project of Shijiazhuang Municipal Science and Technology Bureau(131200063A)Support Program of 100 Outstanding Innovative Talents of Hebei Education Department(ZH2011244)~~
文摘[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups: control group (drug-free group), 1.5% close group, 1.0% dose group and 0.5% dose group. Blood samples of piglets were collected at 20, 35, 50, 60, 70 and 80 days old, respective- ly, to determine neutrophil leukocyte percentage in peripheral blood, lymphocyte trapsformation rate and the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder. [Result] Applying a certain dose of Echinacea compound could significantly increase neutrophil leukocyte per- centage in peripheral blood and lymphocyte transformation rate (P〈0.05), and ex- tremely significantly improve the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder (P〈0.01). [Conclusion] Echi- nacea compound has played a certain role in promoting nonspecific and specific im- mune function of piglets.
文摘[目的]旨在研究中兽药复方紫黄散对免疫抑制小鼠细胞因子的影响。[方法]选取100只昆明系小鼠,利用氢化可的松建立免疫抑制小鼠模型。选取造模成功的小鼠60只,随机分为3组,即模型组、紫黄散组和阳性对照组,分别给予0.25 m L/(10 g·BW·d)的生理盐水、紫黄散口服液及玉屏风口服液,均连续灌胃14 d。另取健康小鼠20只,作为健康对照组。测定并比较给药第0天、第7天、第14天各组小鼠的血液WBC含量及血清中细胞因子IL-2、IL-4、TNF-α和IFN-γ含量。[结果]模型组小鼠给药第0天的血液WBC含量显著低于健康对照组(P<0.05),表明免疫抑制小鼠模型造模成功;给药第7天和第14天,紫黄散组小鼠的血液WBC含量显著高于模型组(P<0.05),且紫黄散组小鼠给药第7天和第14天的血液WBC含量显著高于给药第0天(P<0.05);给药第7天及第14天,紫黄散组小鼠血清IL-2、IL-4、TNF-α和IFN-γ含量显著高于模型组(P<0.05),且与健康对照组及阳性对照组差异不显著(P>0.05)。[结论]紫黄散口服液能够显著提高免疫抑制小鼠的细胞免疫和体液免疫水平,具有一定的免疫增强作用。