为给森林自然教育领域进一步发展提供理论依据,以中国知网(CNKI)和Web of Science(WOS)数据库中2002—2022年间发表的森林自然教育文献为研究对象,采用CiteSpace软件进行发文量、作者、研究机构、国家和关键词分析。结果表明,CNKI和WOS...为给森林自然教育领域进一步发展提供理论依据,以中国知网(CNKI)和Web of Science(WOS)数据库中2002—2022年间发表的森林自然教育文献为研究对象,采用CiteSpace软件进行发文量、作者、研究机构、国家和关键词分析。结果表明,CNKI和WOS数据库中,森林自然教育年度发文量均呈上升趋势;CNKI数据库作者发文量最多的为周彩贤,WOS数据库作者发文量最多的为Berat Ahi;北京林业大学和Chinese Academy of Sciences(中国科学院)发文量均最多;发文量最多的国家为美国(95篇)。CNKI数据库中,森林自然教育发展可分为3个阶段,分别为2002—2008年的基础建设与推广阶段,2009—2015年的教育优化与体验深化阶段和2016—2022年的多元化发展与资源整合阶段。WOS数据库中,森林自然教育发展可分为3个阶段,分别为2002—2008年的基础发展与探索阶段,2009—2015年的深入拓展与多元发展阶段和2016—2022年的全面整合与发展阶段。展开更多
[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to expre...[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase.展开更多
Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ...Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.展开更多
文摘为给森林自然教育领域进一步发展提供理论依据,以中国知网(CNKI)和Web of Science(WOS)数据库中2002—2022年间发表的森林自然教育文献为研究对象,采用CiteSpace软件进行发文量、作者、研究机构、国家和关键词分析。结果表明,CNKI和WOS数据库中,森林自然教育年度发文量均呈上升趋势;CNKI数据库作者发文量最多的为周彩贤,WOS数据库作者发文量最多的为Berat Ahi;北京林业大学和Chinese Academy of Sciences(中国科学院)发文量均最多;发文量最多的国家为美国(95篇)。CNKI数据库中,森林自然教育发展可分为3个阶段,分别为2002—2008年的基础建设与推广阶段,2009—2015年的教育优化与体验深化阶段和2016—2022年的多元化发展与资源整合阶段。WOS数据库中,森林自然教育发展可分为3个阶段,分别为2002—2008年的基础发展与探索阶段,2009—2015年的深入拓展与多元发展阶段和2016—2022年的全面整合与发展阶段。
基金Supported by Science Foundation from Southwest Forestry College(200524M)Natural Science Foundation of Yunan Province(2002C0047M)Key Scientific and Technological Project of Yunan Province(2003NG12)~~
文摘[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase.
文摘Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.
