Preeclampsia（PE） is a pregnancy-specific hypertensive complication,closely related to endothelial dysfunction.Adipose derived stem cells（ADSCs） have the capacity to differentiate into endothelial cells for vascula...Preeclampsia（PE） is a pregnancy-specific hypertensive complication,closely related to endothelial dysfunction.Adipose derived stem cells（ADSCs） have the capacity to differentiate into endothelial cells for vascular repair.Therefore,we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE.In this study,the primary ADSCs and human umbilical vein endothelial cells（HUVECs） were isolated by the collagenase digestion method.The supernatant of HUVECs was collected from the first generation of cells.Then,ADSCs were divided into two groups：ADSCs alone group and induced ADSCs（i ADSCs） group.In i ADSCs group,ADSCs were induced by HUVECs conditioned medium and ADSCs special culture medium at a ratio of 1：1 over a two-week period.In order to identify the endothelial characteristics of i ADSCs,CD31 and CD34 were examined by flow cytometry.The proliferation,migration,invasion and angiogenesis assays were employed to compare the bioactivity of i ADSCs and ADSCs.Furthermore,The levels of angiogenic related factors including vascular endothelial growth factor（VEGF） and placenta growth factor（Pl GF） were detected by RT-PCR and Western blotting.Results showed conditioned medium from HUVECs promoted ADSCs proliferation,migration,invasion and angiogenesis.In addition,the levels of VEGF and Pl GF were significantly enhanced in i ADSCs group.This study uncovered the i ADSCs application potential in the therapy and intervention of PE.展开更多
Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells（EPCs）. Axl was detec...Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells（EPCs）. Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl m RNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy（P〈0.05）. After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.展开更多
基金supported by National Natural Science Foundation of China(No.81100428)
文摘Preeclampsia（PE） is a pregnancy-specific hypertensive complication,closely related to endothelial dysfunction.Adipose derived stem cells（ADSCs） have the capacity to differentiate into endothelial cells for vascular repair.Therefore,we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE.In this study,the primary ADSCs and human umbilical vein endothelial cells（HUVECs） were isolated by the collagenase digestion method.The supernatant of HUVECs was collected from the first generation of cells.Then,ADSCs were divided into two groups：ADSCs alone group and induced ADSCs（i ADSCs） group.In i ADSCs group,ADSCs were induced by HUVECs conditioned medium and ADSCs special culture medium at a ratio of 1：1 over a two-week period.In order to identify the endothelial characteristics of i ADSCs,CD31 and CD34 were examined by flow cytometry.The proliferation,migration,invasion and angiogenesis assays were employed to compare the bioactivity of i ADSCs and ADSCs.Furthermore,The levels of angiogenic related factors including vascular endothelial growth factor（VEGF） and placenta growth factor（Pl GF） were detected by RT-PCR and Western blotting.Results showed conditioned medium from HUVECs promoted ADSCs proliferation,migration,invasion and angiogenesis.In addition,the levels of VEGF and Pl GF were significantly enhanced in i ADSCs group.This study uncovered the i ADSCs application potential in the therapy and intervention of PE.
文摘Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells（EPCs）. Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl m RNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy（P〈0.05）. After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.
