Resonance Light Scattering(RLS) Spectra method is used to study the system of CPC and K2HgI4.In a B-R buffer solution which pH was 2.5,the intensity of CPC’s RLS increased greatly with the formation of ion-associatio...Resonance Light Scattering(RLS) Spectra method is used to study the system of CPC and K2HgI4.In a B-R buffer solution which pH was 2.5,the intensity of CPC’s RLS increased greatly with the formation of ion-association.There was the peak at 497nm which is linear relation to the concentration of CPC.According to that,we built a RLS method of determination of CPC.This method is simple,rapid and sensitive.It was used to determine CPC in the synthetic water samples.The results were satisfactory.展开更多
A new system of FRET was built in this paper.In the condition of λex/λem=400nm/560nm,0.1mol/L Tris buffer(pH=7.0),the effective energy transfer can occur between FITC(Fluorescein 5-isothiocyanate) and Rhodamine 6G(6...A new system of FRET was built in this paper.In the condition of λex/λem=400nm/560nm,0.1mol/L Tris buffer(pH=7.0),the effective energy transfer can occur between FITC(Fluorescein 5-isothiocyanate) and Rhodamine 6G(6G).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of this system liners with BSA from 10mg/L to 35mg/L,and the limit of determination is 9.5mg/L.The linear regression is F=0.0363×CBSA+0.7256 with the correlation coefficient,r=0.9970.展开更多
A new system of FRET was built in this paper.In the condition of λex/λem=480nm/530nm,0.2mol/L HCl,the effective energy transfer can occur between Eosin Y(EY)and Rhodamine B(Rhb).The quenching of fluorescence of the ...A new system of FRET was built in this paper.In the condition of λex/λem=480nm/530nm,0.2mol/L HCl,the effective energy transfer can occur between Eosin Y(EY)and Rhodamine B(Rhb).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of EY-RhB is linear with BSA from 10mg/L to 40mg/L,and the limit of determination is 9.8mg/L.展开更多
文摘Resonance Light Scattering(RLS) Spectra method is used to study the system of CPC and K2HgI4.In a B-R buffer solution which pH was 2.5,the intensity of CPC’s RLS increased greatly with the formation of ion-association.There was the peak at 497nm which is linear relation to the concentration of CPC.According to that,we built a RLS method of determination of CPC.This method is simple,rapid and sensitive.It was used to determine CPC in the synthetic water samples.The results were satisfactory.
文摘A new system of FRET was built in this paper.In the condition of λex/λem=400nm/560nm,0.1mol/L Tris buffer(pH=7.0),the effective energy transfer can occur between FITC(Fluorescein 5-isothiocyanate) and Rhodamine 6G(6G).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of this system liners with BSA from 10mg/L to 35mg/L,and the limit of determination is 9.5mg/L.The linear regression is F=0.0363×CBSA+0.7256 with the correlation coefficient,r=0.9970.
文摘A new system of FRET was built in this paper.In the condition of λex/λem=480nm/530nm,0.2mol/L HCl,the effective energy transfer can occur between Eosin Y(EY)and Rhodamine B(Rhb).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of EY-RhB is linear with BSA from 10mg/L to 40mg/L,and the limit of determination is 9.8mg/L.
