为探究枸杞离散元仿真边界参数的最优组合,提高离散单元仿真方法研究枸杞与采收机械作用机理的准确性,提升后续枸杞机械采收设备的研发效率及机械作业性能,选取EDEM中“Hertz-Mindlin with Johnson-Kendall-Roberts”凝聚力接触模型,通...为探究枸杞离散元仿真边界参数的最优组合,提高离散单元仿真方法研究枸杞与采收机械作用机理的准确性,提升后续枸杞机械采收设备的研发效率及机械作业性能,选取EDEM中“Hertz-Mindlin with Johnson-Kendall-Roberts”凝聚力接触模型,通过物理试验测量枸杞堆积角、枸杞-钢板静摩擦因数、枸杞-钢板碰撞恢复系数。在物理试验的基础上,采用基于EDEMpy的仿真试验方法对枸杞的接触参数进行试验研究,以枸杞颗粒的静摩擦因数、滚动摩擦因数、碰撞恢复系数、表面能JKR为影响因素,以堆积角为目标进行多因素试验,并建立回归模型。试验结果表明:对枸杞仿真堆积角影响显著的参数为枸杞-枸杞静摩擦因数、枸杞-枸杞滚动摩擦因数、表面能JKR。以实际堆积角为目标值,对回归模型进行优化分析,得到显著性参数最优组合值分别为:枸杞-枸杞静摩擦因数0.506,枸杞-枸杞滚动摩擦因数0.064,表面能JKR0.048,该组合下的仿真堆积角平均值为28.82°,与实际堆积角的偏差小于4%,边界参数标定结果可靠。研究结果可为枸杞离散元模型边界参数的选取提供参考。展开更多
目的分离鉴定猪胎盘蛋白。方法十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)切胶处理猪胎盘提取物后,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)对其进行分离鉴定。结果 SDS-PAGE电泳中7条条带最清晰(对应蛋白含有量较高),...目的分离鉴定猪胎盘蛋白。方法十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)切胶处理猪胎盘提取物后,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)对其进行分离鉴定。结果 SDS-PAGE电泳中7条条带最清晰(对应蛋白含有量较高),分子量分别为77.27、68.21、57.85、53.57、46.44、37.16、15.00 k D,分别归属于葡萄糖调节蛋白78、热休克蛋白8、蛋白质二硫键异构酶A3前体蛋白、脯氨酰4-羟化酶β多肽、β细胞骨架肌动蛋白片段、醛糖还原酶、β血红蛋白亚基。结论所首次鉴定出的7个蛋白是猪胎盘增强细胞活力、提高免疫力、抗氧化活性的物质基础,可为相关研究奠定基础。展开更多
Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neurob...Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors.展开更多
文摘为探究枸杞离散元仿真边界参数的最优组合,提高离散单元仿真方法研究枸杞与采收机械作用机理的准确性,提升后续枸杞机械采收设备的研发效率及机械作业性能,选取EDEM中“Hertz-Mindlin with Johnson-Kendall-Roberts”凝聚力接触模型,通过物理试验测量枸杞堆积角、枸杞-钢板静摩擦因数、枸杞-钢板碰撞恢复系数。在物理试验的基础上,采用基于EDEMpy的仿真试验方法对枸杞的接触参数进行试验研究,以枸杞颗粒的静摩擦因数、滚动摩擦因数、碰撞恢复系数、表面能JKR为影响因素,以堆积角为目标进行多因素试验,并建立回归模型。试验结果表明:对枸杞仿真堆积角影响显著的参数为枸杞-枸杞静摩擦因数、枸杞-枸杞滚动摩擦因数、表面能JKR。以实际堆积角为目标值,对回归模型进行优化分析,得到显著性参数最优组合值分别为:枸杞-枸杞静摩擦因数0.506,枸杞-枸杞滚动摩擦因数0.064,表面能JKR0.048,该组合下的仿真堆积角平均值为28.82°,与实际堆积角的偏差小于4%,边界参数标定结果可靠。研究结果可为枸杞离散元模型边界参数的选取提供参考。
文摘目的分离鉴定猪胎盘蛋白。方法十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)切胶处理猪胎盘提取物后,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)对其进行分离鉴定。结果 SDS-PAGE电泳中7条条带最清晰(对应蛋白含有量较高),分子量分别为77.27、68.21、57.85、53.57、46.44、37.16、15.00 k D,分别归属于葡萄糖调节蛋白78、热休克蛋白8、蛋白质二硫键异构酶A3前体蛋白、脯氨酰4-羟化酶β多肽、β细胞骨架肌动蛋白片段、醛糖还原酶、β血红蛋白亚基。结论所首次鉴定出的7个蛋白是猪胎盘增强细胞活力、提高免疫力、抗氧化活性的物质基础,可为相关研究奠定基础。
基金The University Natural Science Research major Project of Jiangsu Province(Grant No.16KJA310006)
文摘Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors.