In higher plant chloroplasts, DNA molecules have inverted repeat sequences about 20--28 kb in length, except for some legume plants--Vicia faba, for example. It has been proved that the recombination between the inver...In higher plant chloroplasts, DNA molecules have inverted repeat sequences about 20--28 kb in length, except for some legume plants--Vicia faba, for example. It has been proved that the recombination between the inverted repeats located on the same or different molecules takes place constantly. It has been proposed that inverted repeats may promote展开更多
The leader sequence of the 16S rDNA gene from the Vicia faba chloroplast is reported in this paper. In this sequence, F1 and the X-protein gene are absent which can be explained by the genome rearrangement. Comparison...The leader sequence of the 16S rDNA gene from the Vicia faba chloroplast is reported in this paper. In this sequence, F1 and the X-protein gene are absent which can be explained by the genome rearrangement. Comparison of F3 of V. faba and those of other plant indicates that: (ⅰ) the conservative regions in F3 of V. faba are more consistent with the consensus sequences, (ⅱ) in F3 of V. faba, the region between --10 and the start point is richer in A-T, and (ⅲ) a stable stem-loop structure can form upstream the 5’ end of the 16S rRNA gene in Brassica napus, tobacco, maize and spinach, however, no such structure could form in V. faba. We conclude that the rDNA promoter of V. faba is more efficient and the single rDNA copy fulfils the role of the double rDNA copies by enhancing the rDNA transcription.展开更多
文摘In higher plant chloroplasts, DNA molecules have inverted repeat sequences about 20--28 kb in length, except for some legume plants--Vicia faba, for example. It has been proved that the recombination between the inverted repeats located on the same or different molecules takes place constantly. It has been proposed that inverted repeats may promote
文摘The leader sequence of the 16S rDNA gene from the Vicia faba chloroplast is reported in this paper. In this sequence, F1 and the X-protein gene are absent which can be explained by the genome rearrangement. Comparison of F3 of V. faba and those of other plant indicates that: (ⅰ) the conservative regions in F3 of V. faba are more consistent with the consensus sequences, (ⅱ) in F3 of V. faba, the region between --10 and the start point is richer in A-T, and (ⅲ) a stable stem-loop structure can form upstream the 5’ end of the 16S rRNA gene in Brassica napus, tobacco, maize and spinach, however, no such structure could form in V. faba. We conclude that the rDNA promoter of V. faba is more efficient and the single rDNA copy fulfils the role of the double rDNA copies by enhancing the rDNA transcription.