A highly selective and rapid anodic adsorptive voltammetric method was developed for the （determination） of ultra trace amount of ofloxacin at a carbon paste electrode（CPE）. The ofloxacin adsorbed on the surface o...A highly selective and rapid anodic adsorptive voltammetric method was developed for the （determination） of ultra trace amount of ofloxacin at a carbon paste electrode（CPE）. The ofloxacin adsorbed on the surface of the CPE in a 0.096 mol/L KHP-NaOH buffer solution（pH=5.6） could produce one oxidation peak at 0.92 V（vs.SCE） when scanning was performed from 0.4 to 1.4 V. The relationship between the peak current and the ofloxacin concentration was linear in the ranges of 5.5×10-9～5.5×10-8 mol/L（75 s accumulation） and 3.3×10-8～3.3×10-7 mol/L（30 s accumulation）. The correlation coefficients were 0.996 and 0.998, respectively. Its detection limit was 9.0×10-10 mol/L（S/N=3） for 150 s accumulation. The （electrode） reaction mechanism of ofloxacin was investigated. The proposed method was applied to analyze trace ofloxacin in urine samples. The result was in good agreement with that obtained by spectrophotometry （determination）.展开更多
A highly selective and rapid new anodic adsorptive voltammetric method was developed for the determination of ultra trace amount of pipemidic acid at a carbon paste electrode（CPE）.The pipemidic acid is adsorbed on t...A highly selective and rapid new anodic adsorptive voltammetric method was developed for the determination of ultra trace amount of pipemidic acid at a carbon paste electrode（CPE）.The pipemidic acid is adsorbed on the surface of the CPE in 0.64mol·L-1NH4Ac-HAc buffer solution（pH4.90）and yields one oxidation peak at 1.05V（vs.SCE）when nearly scanning from 0.8 to 1.8V.The relationship between the peak current and pipemidic acid concentration is linear in the range of 2.0×10-9to 2.0×10-7mol·L-1with the correlation coefficient 0.995.Its detection limit is 6.0×10-10mol·L-1（S/N=3）for 150s accumulation.The proposed method was applied to determine the trace of pipemidic acid in the urine samples.展开更多
文摘A highly selective and rapid anodic adsorptive voltammetric method was developed for the （determination） of ultra trace amount of ofloxacin at a carbon paste electrode（CPE）. The ofloxacin adsorbed on the surface of the CPE in a 0.096 mol/L KHP-NaOH buffer solution（pH=5.6） could produce one oxidation peak at 0.92 V（vs.SCE） when scanning was performed from 0.4 to 1.4 V. The relationship between the peak current and the ofloxacin concentration was linear in the ranges of 5.5×10-9～5.5×10-8 mol/L（75 s accumulation） and 3.3×10-8～3.3×10-7 mol/L（30 s accumulation）. The correlation coefficients were 0.996 and 0.998, respectively. Its detection limit was 9.0×10-10 mol/L（S/N=3） for 150 s accumulation. The （electrode） reaction mechanism of ofloxacin was investigated. The proposed method was applied to analyze trace ofloxacin in urine samples. The result was in good agreement with that obtained by spectrophotometry （determination）.
文摘A highly selective and rapid new anodic adsorptive voltammetric method was developed for the determination of ultra trace amount of pipemidic acid at a carbon paste electrode（CPE）.The pipemidic acid is adsorbed on the surface of the CPE in 0.64mol·L-1NH4Ac-HAc buffer solution（pH4.90）and yields one oxidation peak at 1.05V（vs.SCE）when nearly scanning from 0.8 to 1.8V.The relationship between the peak current and pipemidic acid concentration is linear in the range of 2.0×10-9to 2.0×10-7mol·L-1with the correlation coefficient 0.995.Its detection limit is 6.0×10-10mol·L-1（S/N=3）for 150s accumulation.The proposed method was applied to determine the trace of pipemidic acid in the urine samples.
文摘Wolbachia是广泛分布于昆虫细胞内的一类共生菌,为明确Wolbachia在沟眶象Eucryptorrhynchus chinensis(Olivier)体内的感染情况和分类地位,采用Wolbachia的wsp基因片段对沟眶象5个地理种群(宁夏灵武、中宁、中卫、安徽淮北、山东聊城)共100个样本,进行分子检测。经过克隆和测序得到wsp基因片段593 bp(Gen Bank number:KP713759),明确了沟眶象体内存在Wolbachia的感染,并且其在不同地理种群内的感染率均为100%;通过wsp通用引物检测发现沟眶象体内感染了1种Wolbachia,属于A群Dro亚群。
