In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nod...In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nodulation genes atdownstream begins, when the NodD protein is present. This kind of promoters isvery conservative in structure, always containing nod boxes. In previous reports, the Azorhizobium caulinodans ORS571 as Rhizobium has beenshown to harbour nodABC-related genes (nod locus 1) and nodD-related gene(nodlocus 3). Recently, the details of the identification of a new nod locus 5 gene展开更多
An 800 bp AccI-PUCII DNA containing promoter region of nodABC (P_i)from A. caulinodans ORS571 was cloned and used as a hybridization probe against the chrom(?)some DNA, which led to the identification of another 8.4-k...An 800 bp AccI-PUCII DNA containing promoter region of nodABC (P_i)from A. caulinodans ORS571 was cloned and used as a hybridization probe against the chrom(?)some DNA, which led to the identification of another 8.4-kb EcoRI fragment showing homology to P_1. A corresponding clone of 8.4-kb DNA was isolated from a pLAFI gene bank (pRG90). The restriction enzyme analysis and DNA-DNA hybridization of 8.4-kbDNA indicated the P_1 homology was located in the 450-bp SmaI-SphI region (P_2 region). Ω insertion deletion of 8.4 kb DNA resulted in a mutant strain ORS571-5 that delayed to nodulate the stems of S. rostrata. This phenotype was complemented by the introduction of pRK84 carrying nod locus 5 DNA.展开更多
文摘In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nodulation genes atdownstream begins, when the NodD protein is present. This kind of promoters isvery conservative in structure, always containing nod boxes. In previous reports, the Azorhizobium caulinodans ORS571 as Rhizobium has beenshown to harbour nodABC-related genes (nod locus 1) and nodD-related gene(nodlocus 3). Recently, the details of the identification of a new nod locus 5 gene
文摘An 800 bp AccI-PUCII DNA containing promoter region of nodABC (P_i)from A. caulinodans ORS571 was cloned and used as a hybridization probe against the chrom(?)some DNA, which led to the identification of another 8.4-kb EcoRI fragment showing homology to P_1. A corresponding clone of 8.4-kb DNA was isolated from a pLAFI gene bank (pRG90). The restriction enzyme analysis and DNA-DNA hybridization of 8.4-kbDNA indicated the P_1 homology was located in the 450-bp SmaI-SphI region (P_2 region). Ω insertion deletion of 8.4 kb DNA resulted in a mutant strain ORS571-5 that delayed to nodulate the stems of S. rostrata. This phenotype was complemented by the introduction of pRK84 carrying nod locus 5 DNA.
