对Hall等的数字压力量表(Digital Stress Scale,DSS)进行本土化修订,并检验修订版在国内大学生中的信效度。在样本1(样本量n=15)中访谈数字压力,在4个方便抽样大学生样本中(样本2,3,4和5的n分别为87,100,300和239)对数字压力量表进行项...对Hall等的数字压力量表(Digital Stress Scale,DSS)进行本土化修订,并检验修订版在国内大学生中的信效度。在样本1(样本量n=15)中访谈数字压力,在4个方便抽样大学生样本中(样本2,3,4和5的n分别为87,100,300和239)对数字压力量表进行项目分析、验证性因素分析、结构效度、效标关联效度及信度分析。选取抑郁–焦虑–压力自评量表、生活满意度、UCLA孤独感量表、领悟社会支持和Bergen社交媒体成瘾量表为效标。两周后,对样本5中156名大学生进行重测。修订中文版DSS(Revised Chinese Digital Stress Scale,RC-DSS)中31个条目,包括可联系性压力、认可焦虑、社会比较、错失焦虑、信息过载和在线警戒性6个因子。项目鉴别度和题总分析显示条目表现良好,验证性因素分析显示六因子模型拟合程度良好(χ2/df=2.82,GFI=0.80,NFI=0.93,TLI=0.95,CFI=0.96,RMSEA=0.08)。数字压力与社交媒体成瘾、压力、抑郁、焦虑和孤独感显著正相关(r为0.41~0.61,p都小于0.01),与社会支持和生活满意度显著负相关(r=-0.24,p<0.01;r=-0.15,p<0.05)。量表内部一致性信度为0.94,重测信度为0.73(p<0.01)。RC-DSS具良好的信效度,适用于测量国内大学生的数字压力。展开更多
The researches on chlamydia in recent years show that chlamydia bacteriophage may be a potential and effective means to solve the clinical infection of chlamydia trachomatis（Ct）. We investigated the biological effec...The researches on chlamydia in recent years show that chlamydia bacteriophage may be a potential and effective means to solve the clinical infection of chlamydia trachomatis（Ct）. We investigated the biological effect of chlamydiaphage phi CPG1 capsid protein Vp1 on Ct both in Mc Coy cells and genital tract of mice. Different concentrations of Vp1 were co-incubated with Ct E serotype strain in Mc Coy cells. Female BALB/c mice were used to establish Ct E strain-induced urogenital infection model. They were randomly divided into five groups and given different treatments on the fifth day after Ct inoculation. Animals in groups 1 and 2 were given 30 μL different concentrations of Vp1 in the genital tract respectively, those in group 3 were intramuscularly injected with 30 μL Vp1, those in the infected group did not receive any intervention, and those in the control group received 30 μL PBS in the genital tract. The vaginal discharge was collected to identify the live chlamydia by cell culture and gene fragment by real time PCR different days after infection. Inhibition rate of 100 μg/m L and 50 μg/m L Vp1 proteins against Ct E strain in the Mc Coy cell cultures was 91% and 79% respectively. The number of intracellular Ct inclusion in the Mc Coy cells co-cultured with vaginal discharge of group 1 and group 2 was less than in the infected group, and that in group 1 was less than in group 2, on the 7th day after Ct inoculation. Real-time PCR showed that chlamydia concentration of the vaginal discharge in group 2 was lower than in the infected group, and that in group 1 was lower than in group 2 on the 10 th day. It was suggested that Vp1 capsid proteins had inhibitory effect on the proliferation of Ct serovar E strain in cell culture and mouse genital tract.展开更多
文摘对Hall等的数字压力量表(Digital Stress Scale,DSS)进行本土化修订,并检验修订版在国内大学生中的信效度。在样本1(样本量n=15)中访谈数字压力,在4个方便抽样大学生样本中(样本2,3,4和5的n分别为87,100,300和239)对数字压力量表进行项目分析、验证性因素分析、结构效度、效标关联效度及信度分析。选取抑郁–焦虑–压力自评量表、生活满意度、UCLA孤独感量表、领悟社会支持和Bergen社交媒体成瘾量表为效标。两周后,对样本5中156名大学生进行重测。修订中文版DSS(Revised Chinese Digital Stress Scale,RC-DSS)中31个条目,包括可联系性压力、认可焦虑、社会比较、错失焦虑、信息过载和在线警戒性6个因子。项目鉴别度和题总分析显示条目表现良好,验证性因素分析显示六因子模型拟合程度良好(χ2/df=2.82,GFI=0.80,NFI=0.93,TLI=0.95,CFI=0.96,RMSEA=0.08)。数字压力与社交媒体成瘾、压力、抑郁、焦虑和孤独感显著正相关(r为0.41~0.61,p都小于0.01),与社会支持和生活满意度显著负相关(r=-0.24,p<0.01;r=-0.15,p<0.05)。量表内部一致性信度为0.94,重测信度为0.73(p<0.01)。RC-DSS具良好的信效度,适用于测量国内大学生的数字压力。
基金supported by National Natural Science Foundation of China(No.31370211)
文摘The researches on chlamydia in recent years show that chlamydia bacteriophage may be a potential and effective means to solve the clinical infection of chlamydia trachomatis（Ct）. We investigated the biological effect of chlamydiaphage phi CPG1 capsid protein Vp1 on Ct both in Mc Coy cells and genital tract of mice. Different concentrations of Vp1 were co-incubated with Ct E serotype strain in Mc Coy cells. Female BALB/c mice were used to establish Ct E strain-induced urogenital infection model. They were randomly divided into five groups and given different treatments on the fifth day after Ct inoculation. Animals in groups 1 and 2 were given 30 μL different concentrations of Vp1 in the genital tract respectively, those in group 3 were intramuscularly injected with 30 μL Vp1, those in the infected group did not receive any intervention, and those in the control group received 30 μL PBS in the genital tract. The vaginal discharge was collected to identify the live chlamydia by cell culture and gene fragment by real time PCR different days after infection. Inhibition rate of 100 μg/m L and 50 μg/m L Vp1 proteins against Ct E strain in the Mc Coy cell cultures was 91% and 79% respectively. The number of intracellular Ct inclusion in the Mc Coy cells co-cultured with vaginal discharge of group 1 and group 2 was less than in the infected group, and that in group 1 was less than in group 2, on the 7th day after Ct inoculation. Real-time PCR showed that chlamydia concentration of the vaginal discharge in group 2 was lower than in the infected group, and that in group 1 was lower than in group 2 on the 10 th day. It was suggested that Vp1 capsid proteins had inhibitory effect on the proliferation of Ct serovar E strain in cell culture and mouse genital tract.
