Objective: To diagnose prostatic cancer (CaP) with cDNA macroarray. Methods: Total RNA was isolated from patients with prostate cancer and from normal people, and poly(A) RNA was further purified. Then differentially ...Objective: To diagnose prostatic cancer (CaP) with cDNA macroarray. Methods: Total RNA was isolated from patients with prostate cancer and from normal people, and poly(A) RNA was further purified. Then differentially expressed genes were analysed in CaP and normal prostate by cDNA macroarray system. Results: There were differential expressions of nine prostate-associated specific genes in CaP as compared with normal prostate, among which, 7 were significantly up-regulated and 2 were down-regulated. Conclusion: As a diagnostic approach at molecular level, the cDNA macroarray is supposed to elevate the detection rate of CaP.展开更多
Objective： To assess the effect of transrectal ultrasound guided prostatic nerve blockade on the discomfort associated with systematic biopsy of the prostate. Methods： 73 patients receiving systematic 13 cores biops...Objective： To assess the effect of transrectal ultrasound guided prostatic nerve blockade on the discomfort associated with systematic biopsy of the prostate. Methods： 73 patients receiving systematic 13 cores biopsy of the prostate were randomized into two groups. Group A（37 cases） received an injection of 5 ml 1% lidocaine into the prostatic neurovascular bundles on each side at the base of the prostate under ultrasound guidance and group B（36 cases） received 5 ml saline injection （0.9% sodium chloride） at the same site. Pain during biopsy was assessed by using a 10-point linear visual analog score （VAS） immediately after the biopsy. Results： The mean pain scores during transrectal prostate biopsy were significantly lower in group A than group B（1.1±0.6 versus 5.9±3.1, t=4.81, P〈0.01）. During this study no patient in either group had any adverse effect from the injection. Conclusion： Transcrectal ultrasound guided prostatic nerve blockade is a safe and efficacious method for providing satisfactory anesthesia in transrectal prostate biopsy. We recommend its routine administration in all patients during this procedure.展开更多
文摘Objective: To diagnose prostatic cancer (CaP) with cDNA macroarray. Methods: Total RNA was isolated from patients with prostate cancer and from normal people, and poly(A) RNA was further purified. Then differentially expressed genes were analysed in CaP and normal prostate by cDNA macroarray system. Results: There were differential expressions of nine prostate-associated specific genes in CaP as compared with normal prostate, among which, 7 were significantly up-regulated and 2 were down-regulated. Conclusion: As a diagnostic approach at molecular level, the cDNA macroarray is supposed to elevate the detection rate of CaP.
文摘Objective： To assess the effect of transrectal ultrasound guided prostatic nerve blockade on the discomfort associated with systematic biopsy of the prostate. Methods： 73 patients receiving systematic 13 cores biopsy of the prostate were randomized into two groups. Group A（37 cases） received an injection of 5 ml 1% lidocaine into the prostatic neurovascular bundles on each side at the base of the prostate under ultrasound guidance and group B（36 cases） received 5 ml saline injection （0.9% sodium chloride） at the same site. Pain during biopsy was assessed by using a 10-point linear visual analog score （VAS） immediately after the biopsy. Results： The mean pain scores during transrectal prostate biopsy were significantly lower in group A than group B（1.1±0.6 versus 5.9±3.1, t=4.81, P〈0.01）. During this study no patient in either group had any adverse effect from the injection. Conclusion： Transcrectal ultrasound guided prostatic nerve blockade is a safe and efficacious method for providing satisfactory anesthesia in transrectal prostate biopsy. We recommend its routine administration in all patients during this procedure.
