WNT(wingless-type MMTV integration site family)蛋白是一类分泌性糖蛋白家族,Wnt5a为非经典Wnt蛋白的典型代表,在各种器官的发育中发挥重要作用。为了探索东亚三角涡虫Dj Wnt5a基因在涡虫胚胎不同发育阶段中的表达和功能,利用RT-PCR(...WNT(wingless-type MMTV integration site family)蛋白是一类分泌性糖蛋白家族,Wnt5a为非经典Wnt蛋白的典型代表,在各种器官的发育中发挥重要作用。为了探索东亚三角涡虫Dj Wnt5a基因在涡虫胚胎不同发育阶段中的表达和功能,利用RT-PCR(reverse transcription PCR)和RACE(rapid-amplification of c DNA ends)技术克隆并分析了Dj Wnt5a c DNA的表达。结果表明,Dj Wnt5a基因由1 523 bp组成,编码371个氨基酸,其中含有23个保守的半胱氨酸结构域。利用MAGE 5.2构建进化树分析发现,东亚三角涡虫与地中海涡虫的Wnt5a聚群,并位于两侧对称动物的基部,可能是Wnt5a的原始类型。利用整体原位杂交技术分析Dj Wnt5a基因的表达,结果显示,Dj Wnt5a基因在涡虫发育的第三阶段开始表达,主要集中在胚胎咽及胚带;随着胚胎发育直至完全孵化出幼虫,Dj Wnt5a基因逐步在神经原基和中枢神经系统表达。由此推断,东亚三角涡虫Dj Wnt5a基因在涡虫胚胎发育时,参与神经原基的形成和分化,是涡虫胚胎神经发育必不可少的基因之一。展开更多
Focusing on the origin of the heavy metal,this study aims to build an imputed method to estimate the heavy metal content in river water by making a distinction between the heavy metal of natural origins and that cause...Focusing on the origin of the heavy metal,this study aims to build an imputed method to estimate the heavy metal content in river water by making a distinction between the heavy metal of natural origins and that caused by human activities.Supported by GIS,Kosaka watershed within the Hokuroku basin was divided into several sub-watershed polygons and the outflows of water and Cu were calculated for each polygon.Compared with the natural origin,the dominant Cu emissions affected the river water more significantly in local.Based on the mass balance closure,the heavy metal content of Cu in the Kosaka River was estimated by the conflux accumulation of tributaries and mine drainages.The estimated Cu concentrations were checked by comparing with the actually measured values at monitoring points along the Kosaka River and the results are coincidence with each other in general.It is revealed that the mainstream water quality could be estimated by seizing the water quality of upstream tributaries and human drainages.展开更多
文摘WNT(wingless-type MMTV integration site family)蛋白是一类分泌性糖蛋白家族,Wnt5a为非经典Wnt蛋白的典型代表,在各种器官的发育中发挥重要作用。为了探索东亚三角涡虫Dj Wnt5a基因在涡虫胚胎不同发育阶段中的表达和功能,利用RT-PCR(reverse transcription PCR)和RACE(rapid-amplification of c DNA ends)技术克隆并分析了Dj Wnt5a c DNA的表达。结果表明,Dj Wnt5a基因由1 523 bp组成,编码371个氨基酸,其中含有23个保守的半胱氨酸结构域。利用MAGE 5.2构建进化树分析发现,东亚三角涡虫与地中海涡虫的Wnt5a聚群,并位于两侧对称动物的基部,可能是Wnt5a的原始类型。利用整体原位杂交技术分析Dj Wnt5a基因的表达,结果显示,Dj Wnt5a基因在涡虫发育的第三阶段开始表达,主要集中在胚胎咽及胚带;随着胚胎发育直至完全孵化出幼虫,Dj Wnt5a基因逐步在神经原基和中枢神经系统表达。由此推断,东亚三角涡虫Dj Wnt5a基因在涡虫胚胎发育时,参与神经原基的形成和分化,是涡虫胚胎神经发育必不可少的基因之一。
文摘Focusing on the origin of the heavy metal,this study aims to build an imputed method to estimate the heavy metal content in river water by making a distinction between the heavy metal of natural origins and that caused by human activities.Supported by GIS,Kosaka watershed within the Hokuroku basin was divided into several sub-watershed polygons and the outflows of water and Cu were calculated for each polygon.Compared with the natural origin,the dominant Cu emissions affected the river water more significantly in local.Based on the mass balance closure,the heavy metal content of Cu in the Kosaka River was estimated by the conflux accumulation of tributaries and mine drainages.The estimated Cu concentrations were checked by comparing with the actually measured values at monitoring points along the Kosaka River and the results are coincidence with each other in general.It is revealed that the mainstream water quality could be estimated by seizing the water quality of upstream tributaries and human drainages.
