在实施乡村振兴战略的大背景下,广西乡村旅游业面临转型升级的迫切要求,文章旨在建立促进广西乡村旅游产业转型升级的评价指标体系,并通过明确指导思想制定原则进行考核评价工作。研究以产业自身发展状况外部环境和功能效益三个层面进...在实施乡村振兴战略的大背景下,广西乡村旅游业面临转型升级的迫切要求,文章旨在建立促进广西乡村旅游产业转型升级的评价指标体系,并通过明确指导思想制定原则进行考核评价工作。研究以产业自身发展状况外部环境和功能效益三个层面进行综合考核,形成智慧旅游产业融合提质增效文化推广环境保护等五个方面的评价指标体系,以促进广西乡村旅游产业转型升级。该评价指标体系是当前实施乡村振兴战略中可以借鉴的,为乡村旅游产业的转型升级提供了有力支撑,对促进广西农村振兴提供了科学决策的参考依据。Under the background of the implementation of the rural revitalization strategy, rural tourism in Guangxi is facing the urgent requirement of transformation and upgrading. This paper aims to establish an evaluation index system to promote the transformation and upgrading of rural tourism industry in Guangxi, and carry out assessment and evaluation through clear guiding ideology and formulation principles. This study conducted a comprehensive assessment on the industry’s own development status, external environment and functional benefits, and formed an evaluation index system from five aspects, including the integration, improvement and efficiency of the smart tourism industry, cultural promotion and environmental protection, so as to promote the transformation and upgrading of the rural tourism industry in Guangxi. This evaluation index system can be used for reference in the current implementation of the rural revitalization strategy, which provides strong support for the transformation and upgrading of the rural tourism industry and provides a scientific decision-making basis for promoting the rural revitalization of Guangxi.展开更多
A new method for the preparation of oligonucleotide microarray for gene expression detection was found. The key techniques and standards of quality controlling for preparation of oligonucleotide microarray was explore...A new method for the preparation of oligonucleotide microarray for gene expression detection was found. The key techniques and standards of quality controlling for preparation of oligonucleotide microarray was explored using gene of human 23kD highly protein and Luciferase and mouse cytokine-associated genes. By the using of a software system MProbe, oligonucleotide probes were designed and BLAST. All the probes have a very high specificity, i.e. except target sequence, the similarity between the probe and non-target sequences is less than 70% and the hairpin structure are not exist in all probes.All the probes have the same length 40. GC contents in all probes are in a narrow scope (from 45% to 55%). All the probes are modified with amino at 5′ or 3′ terminal. The satisfied images with good sensativity and very high specificity were obtained by the using of the methods above and also using of positive and negative controls and some internal controls(house keeping gene) to quantitate and balance expression of genes. High specificity, good sensativity and stablity have been verified by three continuous experiments using the oligonucleotide microarray to study gene expression profile of normal mouse breast grand tissue .The oligonucleotide microarray for expression detection prepared using our method have high specificity, good sensativity and stablity et al . It may be a more advanced method for analysis of gene expression profile.展开更多
文摘在实施乡村振兴战略的大背景下,广西乡村旅游业面临转型升级的迫切要求,文章旨在建立促进广西乡村旅游产业转型升级的评价指标体系,并通过明确指导思想制定原则进行考核评价工作。研究以产业自身发展状况外部环境和功能效益三个层面进行综合考核,形成智慧旅游产业融合提质增效文化推广环境保护等五个方面的评价指标体系,以促进广西乡村旅游产业转型升级。该评价指标体系是当前实施乡村振兴战略中可以借鉴的,为乡村旅游产业的转型升级提供了有力支撑,对促进广西农村振兴提供了科学决策的参考依据。Under the background of the implementation of the rural revitalization strategy, rural tourism in Guangxi is facing the urgent requirement of transformation and upgrading. This paper aims to establish an evaluation index system to promote the transformation and upgrading of rural tourism industry in Guangxi, and carry out assessment and evaluation through clear guiding ideology and formulation principles. This study conducted a comprehensive assessment on the industry’s own development status, external environment and functional benefits, and formed an evaluation index system from five aspects, including the integration, improvement and efficiency of the smart tourism industry, cultural promotion and environmental protection, so as to promote the transformation and upgrading of the rural tourism industry in Guangxi. This evaluation index system can be used for reference in the current implementation of the rural revitalization strategy, which provides strong support for the transformation and upgrading of the rural tourism industry and provides a scientific decision-making basis for promoting the rural revitalization of Guangxi.
文摘A new method for the preparation of oligonucleotide microarray for gene expression detection was found. The key techniques and standards of quality controlling for preparation of oligonucleotide microarray was explored using gene of human 23kD highly protein and Luciferase and mouse cytokine-associated genes. By the using of a software system MProbe, oligonucleotide probes were designed and BLAST. All the probes have a very high specificity, i.e. except target sequence, the similarity between the probe and non-target sequences is less than 70% and the hairpin structure are not exist in all probes.All the probes have the same length 40. GC contents in all probes are in a narrow scope (from 45% to 55%). All the probes are modified with amino at 5′ or 3′ terminal. The satisfied images with good sensativity and very high specificity were obtained by the using of the methods above and also using of positive and negative controls and some internal controls(house keeping gene) to quantitate and balance expression of genes. High specificity, good sensativity and stablity have been verified by three continuous experiments using the oligonucleotide microarray to study gene expression profile of normal mouse breast grand tissue .The oligonucleotide microarray for expression detection prepared using our method have high specificity, good sensativity and stablity et al . It may be a more advanced method for analysis of gene expression profile.
