针对突出煤层工作面预抽钻孔预抽浓度低、钻孔衰减系数大、瓦斯预抽时间长等难题,以某煤矿111805工作面为研究对象。通过对该工作面地质条件、瓦斯赋存状态及开采工艺的综合分析,对111805工作面钻孔布孔方式以及管径最优参数进行研究,...针对突出煤层工作面预抽钻孔预抽浓度低、钻孔衰减系数大、瓦斯预抽时间长等难题,以某煤矿111805工作面为研究对象。通过对该工作面地质条件、瓦斯赋存状态及开采工艺的综合分析,对111805工作面钻孔布孔方式以及管径最优参数进行研究,并利用数值模拟软件进行辅证。结果表明:顺层钻孔抽采瓦斯在30 d后有效抽采半径为2.33 m,50 d后有效抽采半径为3 m。对模拟数据进行回归分析,得到的抽采有效半径r与抽采时间t的线性拟合关系,通过结合实际分析,确定抽采钻孔方式为顺层钻孔设计,布置间距约为4 m,瓦斯抽采管径为Ф200 mm抽采管连网抽采。以此为依据,得出工作面瓦斯抽采钻孔最佳布置参数。研究成果为突出煤层工作面预抽钻孔设计提供了参考依据。In response to the challenges of low pre-drilling pre-extraction concentration, large drilling decay coefficient, and long gas pre-extraction time in the working face of outburst-prone coal seams, a study was conducted with the 111805 working face of a coal mine as the research object. Through a comprehensive analysis of the geological conditions, gas occurrence state, and mining technology of the working face, the optimal parameters of drilling layout and pipe diameter for the 111805 working face were studied, and numerical simulation software was used for auxiliary verification. The results showed that the effective extraction radius of the gas extraction from the in-seam drilling was 2.33 meters after 30 days and 3 meters after 50 days. Through regression analysis of the simulation data, a linear fitting relationship between the effective extraction radius r and the extraction time t was obtained. By combining with practical analysis, the drilling method was determined to be in-seam drilling design, with a layout spacing of approximately 4 meters and a gas extraction pipe diameter of 200 mm connected in a network for extraction. Based on this, the optimal layout parameters for gas extraction drilling in the working face were derived. The research results provide a reference for the design of pre-drilling in outburst-prone coal seam working faces.展开更多
为建立山羊IL-2基因SYBR GREEN I实时荧光定量RT-PCR检测方法，根据GenBank中山羊见-2基因（登录号：KT934548），设计1对特异性引物，用于扩增目的基因，并将目的基因克隆于pMD-19-T载体，转化至大肠杆菌DH50L，经质粒PCR及序列测定鉴...为建立山羊IL-2基因SYBR GREEN I实时荧光定量RT-PCR检测方法，根据GenBank中山羊见-2基因（登录号：KT934548），设计1对特异性引物，用于扩增目的基因，并将目的基因克隆于pMD-19-T载体，转化至大肠杆菌DH50L，经质粒PCR及序列测定鉴定后获得阳性重组质粒，作为标准品模板建立SYBR GREEN I RTFQ-PCR标准曲线和溶解曲线，进行特异性、重复性和敏感性试验。并应用所建立的方法，检测ConA刺激健康山羊PBMC后0h、2h、4h、6h、8h、10h、12h、24h和48h不同时间点IL-2基因转录的动态变化。结果表明，当质粒标准品稀释度在7．2×10^9-7．2×10^5copies／μL扩增曲线的Ct值与浓度间具有良好的线性关系，相关系数为一0．996；熔解曲线为特异性单峰，组内变异系数为0．306％～1．458％，组间变异系数为0．514％～1．191％，最低检测限为7．2×10^2copies／μL。山羊IL-2基因的mRNA转录量在0～2h呈现上升趋势，在2h达到峰值，2-6h呈现下降趋势。6～12h未能检测到IL-2基因的mRNA转录；12～48h检测到IL-2基因的mRNA转录量呈逐渐上升趋势。研究结果将为山羊IL-2基因的定量分析提供技术平台。展开更多
文摘针对突出煤层工作面预抽钻孔预抽浓度低、钻孔衰减系数大、瓦斯预抽时间长等难题,以某煤矿111805工作面为研究对象。通过对该工作面地质条件、瓦斯赋存状态及开采工艺的综合分析,对111805工作面钻孔布孔方式以及管径最优参数进行研究,并利用数值模拟软件进行辅证。结果表明:顺层钻孔抽采瓦斯在30 d后有效抽采半径为2.33 m,50 d后有效抽采半径为3 m。对模拟数据进行回归分析,得到的抽采有效半径r与抽采时间t的线性拟合关系,通过结合实际分析,确定抽采钻孔方式为顺层钻孔设计,布置间距约为4 m,瓦斯抽采管径为Ф200 mm抽采管连网抽采。以此为依据,得出工作面瓦斯抽采钻孔最佳布置参数。研究成果为突出煤层工作面预抽钻孔设计提供了参考依据。In response to the challenges of low pre-drilling pre-extraction concentration, large drilling decay coefficient, and long gas pre-extraction time in the working face of outburst-prone coal seams, a study was conducted with the 111805 working face of a coal mine as the research object. Through a comprehensive analysis of the geological conditions, gas occurrence state, and mining technology of the working face, the optimal parameters of drilling layout and pipe diameter for the 111805 working face were studied, and numerical simulation software was used for auxiliary verification. The results showed that the effective extraction radius of the gas extraction from the in-seam drilling was 2.33 meters after 30 days and 3 meters after 50 days. Through regression analysis of the simulation data, a linear fitting relationship between the effective extraction radius r and the extraction time t was obtained. By combining with practical analysis, the drilling method was determined to be in-seam drilling design, with a layout spacing of approximately 4 meters and a gas extraction pipe diameter of 200 mm connected in a network for extraction. Based on this, the optimal layout parameters for gas extraction drilling in the working face were derived. The research results provide a reference for the design of pre-drilling in outburst-prone coal seam working faces.
文摘为建立山羊IL-2基因SYBR GREEN I实时荧光定量RT-PCR检测方法，根据GenBank中山羊见-2基因（登录号：KT934548），设计1对特异性引物，用于扩增目的基因，并将目的基因克隆于pMD-19-T载体，转化至大肠杆菌DH50L，经质粒PCR及序列测定鉴定后获得阳性重组质粒，作为标准品模板建立SYBR GREEN I RTFQ-PCR标准曲线和溶解曲线，进行特异性、重复性和敏感性试验。并应用所建立的方法，检测ConA刺激健康山羊PBMC后0h、2h、4h、6h、8h、10h、12h、24h和48h不同时间点IL-2基因转录的动态变化。结果表明，当质粒标准品稀释度在7．2×10^9-7．2×10^5copies／μL扩增曲线的Ct值与浓度间具有良好的线性关系，相关系数为一0．996；熔解曲线为特异性单峰，组内变异系数为0．306％～1．458％，组间变异系数为0．514％～1．191％，最低检测限为7．2×10^2copies／μL。山羊IL-2基因的mRNA转录量在0～2h呈现上升趋势，在2h达到峰值，2-6h呈现下降趋势。6～12h未能检测到IL-2基因的mRNA转录；12～48h检测到IL-2基因的mRNA转录量呈逐渐上升趋势。研究结果将为山羊IL-2基因的定量分析提供技术平台。
