Free radical induced neuronal damage is involved in stroke. Several in vitro and in vivo studies have proved that antioxidant could act as neuroprotective agent through intervening with flee-radical mediated apoptosis...Free radical induced neuronal damage is involved in stroke. Several in vitro and in vivo studies have proved that antioxidant could act as neuroprotective agent through intervening with flee-radical mediated apoptosis in the ischemic penumbra. In particular, natural products which contain antioxidant properties have undoubtedly potential for stroke treatment. In the present study, therapeutic effects of Braintone on Wistar rats undergone middle cerebral artery occlusion （MCAO） was evaluated. Gene expression levels of pro-apoptotic genes （AT2 receptor, FAS, BAX and BCL-XS） were showed to be significantly reduced in Braintone treated groups （0.4-, 0.72-, 0.76-, 0.32-fold, P〈0.05） as compared to vehicle group. Significant reduction ofimmunoreactivity of protein production of these genes, together with least nuclear green fluorescence observed in TUNEL, Braintone as an antioxidant drug, is concluded to have promising therapeutic effect for stroke treatment.展开更多
基金National Medical Research Council of Singapore (R-184-000-037-213 and R-184-000-109-213)
文摘Free radical induced neuronal damage is involved in stroke. Several in vitro and in vivo studies have proved that antioxidant could act as neuroprotective agent through intervening with flee-radical mediated apoptosis in the ischemic penumbra. In particular, natural products which contain antioxidant properties have undoubtedly potential for stroke treatment. In the present study, therapeutic effects of Braintone on Wistar rats undergone middle cerebral artery occlusion （MCAO） was evaluated. Gene expression levels of pro-apoptotic genes （AT2 receptor, FAS, BAX and BCL-XS） were showed to be significantly reduced in Braintone treated groups （0.4-, 0.72-, 0.76-, 0.32-fold, P〈0.05） as compared to vehicle group. Significant reduction ofimmunoreactivity of protein production of these genes, together with least nuclear green fluorescence observed in TUNEL, Braintone as an antioxidant drug, is concluded to have promising therapeutic effect for stroke treatment.
