利用特异性的引物对 ,选择性扩增垃圾填埋场渗滤液中古细菌群落的 1 6 S r RNA基因片断 ,在此基础上建立 1 6 Sr DNA克隆文库。经古细菌通用寡核苷酸探针的原位杂交筛选后 ,克隆文库内古细菌 1 6 S r DNA扩增片断的多样性通过ARDRA分析 ...利用特异性的引物对 ,选择性扩增垃圾填埋场渗滤液中古细菌群落的 1 6 S r RNA基因片断 ,在此基础上建立 1 6 Sr DNA克隆文库。经古细菌通用寡核苷酸探针的原位杂交筛选后 ,克隆文库内古细菌 1 6 S r DNA扩增片断的多样性通过ARDRA分析 ( amplified r DNA restriction analysis)而获得。利用 PCR将各重组克隆子内的 1 6 S r DNA外源片断再扩增出来后 ,两种限制性内切酶 -H ha 和 H ae -被分别用于 1 6 Sr DNA克隆片断的限制酶切分析。结果表明 ,随机选出的 70个古细菌 1 6 S r DNA克隆片断被分为 2 1个不同的 ARDRA型 (组 ) ,其中的两个优势型总共占了所有被分析克隆子的6 0 % ,而其余 1 9个型的相对丰度均处于较低的水平 ,当中的 1 4个型更仅含有 1个克隆子。通过对 1 6 Sr RNA基因的 PCR扩增、克隆及其 ARDRA分析 。展开更多
In order to study the feasibility of using mangrove wetland as a facility for sewage treatment and the effects of wastewater discharge on soil and plants of the mangrove ecosystem,the field work was conducted during D...In order to study the feasibility of using mangrove wetland as a facility for sewage treatment and the effects of wastewater discharge on soil and plants of the mangrove ecosystem,the field work was conducted during December 1994 to December 1995 in a mangrove wetland in Futian national nature reserve in Shenzhen. Two parallel elongated sites (sites A and B, each 180m×10m) stretching from land to sea were chosen for study. Since December 1994, Site A was continuously irrigated with municipal sewage every two days,while Site B served as a control. The ecological impact of sewage discharge on the mangrove wetland was assessed by comparing the plant growth, nutrients of soils and vegetation between Site A and Site B. One year later, total-N and NH 4 +-N in the soil increased and EC decreased significantly at Site A. And obvious changes in pH value and other nutrient contents in the soil of Site A could be detected. However, the nutrient contents of the leaves of the dominant plants collected from Site A and B were very close and no significant difference in terms of tree height and diameter and biomass was found between the two sites.展开更多
文摘利用特异性的引物对 ,选择性扩增垃圾填埋场渗滤液中古细菌群落的 1 6 S r RNA基因片断 ,在此基础上建立 1 6 Sr DNA克隆文库。经古细菌通用寡核苷酸探针的原位杂交筛选后 ,克隆文库内古细菌 1 6 S r DNA扩增片断的多样性通过ARDRA分析 ( amplified r DNA restriction analysis)而获得。利用 PCR将各重组克隆子内的 1 6 S r DNA外源片断再扩增出来后 ,两种限制性内切酶 -H ha 和 H ae -被分别用于 1 6 Sr DNA克隆片断的限制酶切分析。结果表明 ,随机选出的 70个古细菌 1 6 S r DNA克隆片断被分为 2 1个不同的 ARDRA型 (组 ) ,其中的两个优势型总共占了所有被分析克隆子的6 0 % ,而其余 1 9个型的相对丰度均处于较低的水平 ,当中的 1 4个型更仅含有 1个克隆子。通过对 1 6 Sr RNA基因的 PCR扩增、克隆及其 ARDRA分析 。
文摘In order to study the feasibility of using mangrove wetland as a facility for sewage treatment and the effects of wastewater discharge on soil and plants of the mangrove ecosystem,the field work was conducted during December 1994 to December 1995 in a mangrove wetland in Futian national nature reserve in Shenzhen. Two parallel elongated sites (sites A and B, each 180m×10m) stretching from land to sea were chosen for study. Since December 1994, Site A was continuously irrigated with municipal sewage every two days,while Site B served as a control. The ecological impact of sewage discharge on the mangrove wetland was assessed by comparing the plant growth, nutrients of soils and vegetation between Site A and Site B. One year later, total-N and NH 4 +-N in the soil increased and EC decreased significantly at Site A. And obvious changes in pH value and other nutrient contents in the soil of Site A could be detected. However, the nutrient contents of the leaves of the dominant plants collected from Site A and B were very close and no significant difference in terms of tree height and diameter and biomass was found between the two sites.
