Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods: The models of abdominal aorta transplantation were made with micro-surgery i...Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups were observed by histological examination. The expression of PCNA and α-SMA were determined by immunohistochemistry. The content of nitric oxide was determined by nitrate reductase chromatometry. Results: The vascular intimal thickness in rats of atorvastatin-treated group (11.60% ± 2.40% ) were lower than those in allograft control group (34.60 % ± 6.40 % ; P 〈 0.05) and higher than those in isograft control group (1.15 % ± 0.65 %; P〈 0.05 ). The expression level of PCNA was decreased in atorvastatin-treated group (4.80% ± 0.80% ) than allograft control group (18.40% ± 1.80% ; P〈0.05) and higher than isograft group (1.20% ± 0.40% ; P〈0.05). Conclusion: The expression of PCNA in the transplant aorta could be suppressed by atorvastatin, which resalted in relief of chronic rejection of aortic allograft.展开更多
文摘Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups were observed by histological examination. The expression of PCNA and α-SMA were determined by immunohistochemistry. The content of nitric oxide was determined by nitrate reductase chromatometry. Results: The vascular intimal thickness in rats of atorvastatin-treated group (11.60% ± 2.40% ) were lower than those in allograft control group (34.60 % ± 6.40 % ; P 〈 0.05) and higher than those in isograft control group (1.15 % ± 0.65 %; P〈 0.05 ). The expression level of PCNA was decreased in atorvastatin-treated group (4.80% ± 0.80% ) than allograft control group (18.40% ± 1.80% ; P〈0.05) and higher than isograft group (1.20% ± 0.40% ; P〈0.05). Conclusion: The expression of PCNA in the transplant aorta could be suppressed by atorvastatin, which resalted in relief of chronic rejection of aortic allograft.