目的通过文献计量学方法对近十年Web of Science数据库中收录的法庭科学相关文献进行分析,为相关研究工作提供借鉴。方法使用主题、期刊和机构3种途径进行文献检索,通过Thomson Data Analyzer(TDA)、Ucinet和VOSviewer等工具分析相应文...目的通过文献计量学方法对近十年Web of Science数据库中收录的法庭科学相关文献进行分析,为相关研究工作提供借鉴。方法使用主题、期刊和机构3种途径进行文献检索,通过Thomson Data Analyzer(TDA)、Ucinet和VOSviewer等工具分析相应文献的年度分布、国家(地区)分布、机构分布、期刊分布和研究热点。结果近十年,相应文献共计49469篇。发文量呈逐年上升趋势。发文量前15的国家(地区)占发文总量的78.52%;中国发文量位列第5,但高被引文献所占比例位列第12;荷兰、瑞士和澳大利亚等国家(地区)综合影响力较高。中国合作国家(地区)主要包括美国、英国和德国等129个。圣玛丽大学法庭科学学院、悉尼大学和荷兰法医协会等综合影响力较高,相应文献发表在6357种期刊。根据领域高频词共现网络和高被引文献遴选出颅脑损伤、医疗卫生政策、评定量表和模型、医学影像学等研究热点。结论国际法庭科学领域SCI收录文献总数增速明显,我国成果影响力需大力提升;国际研究机构分散,我国研究力量需不断凝聚;国际领域研究热点广泛,我国高水平研究的国际参与度需继续提高。展开更多
The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested...The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.展开更多
文摘目的通过文献计量学方法对近十年Web of Science数据库中收录的法庭科学相关文献进行分析,为相关研究工作提供借鉴。方法使用主题、期刊和机构3种途径进行文献检索,通过Thomson Data Analyzer(TDA)、Ucinet和VOSviewer等工具分析相应文献的年度分布、国家(地区)分布、机构分布、期刊分布和研究热点。结果近十年,相应文献共计49469篇。发文量呈逐年上升趋势。发文量前15的国家(地区)占发文总量的78.52%;中国发文量位列第5,但高被引文献所占比例位列第12;荷兰、瑞士和澳大利亚等国家(地区)综合影响力较高。中国合作国家(地区)主要包括美国、英国和德国等129个。圣玛丽大学法庭科学学院、悉尼大学和荷兰法医协会等综合影响力较高,相应文献发表在6357种期刊。根据领域高频词共现网络和高被引文献遴选出颅脑损伤、医疗卫生政策、评定量表和模型、医学影像学等研究热点。结论国际法庭科学领域SCI收录文献总数增速明显,我国成果影响力需大力提升;国际研究机构分散,我国研究力量需不断凝聚;国际领域研究热点广泛,我国高水平研究的国际参与度需继续提高。
基金supported by grants from the National Natural Science Foundation of China(No.81102304)Doctoral Fund of Ministry of Education of China(No.20090142120054)Foundation of Huazhong University of Science and Technology(No.20100573)
文摘The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
