The sterile seedlings of Hibiscus esculentus L.were obtained via sterile germination.The stem segments with one leaf and one axillary bud could be repeatedly subcultured on the medium"MS+1 0?mg/L IBA", and a...The sterile seedlings of Hibiscus esculentus L.were obtained via sterile germination.The stem segments with one leaf and one axillary bud could be repeatedly subcultured on the medium"MS+1 0?mg/L IBA", and a propagation coefficient is 3-4 in each subculture. KT had a strong inhibiting effect on the germination and growth of the axillary buds of Hibiscus esculentus L., and a strong dedifferentiating effect on the stem segments. About 25% of the calli, cultured on the medium"MS+0 5-1 0?mg/L IBA" for 50-60 days,transformed into somatic embryoids, which were visible to the naked eye. Most of the somatic embryoids will develope into the intact plantlets on the same medium 30 days later.展开更多
文摘The sterile seedlings of Hibiscus esculentus L.were obtained via sterile germination.The stem segments with one leaf and one axillary bud could be repeatedly subcultured on the medium"MS+1 0?mg/L IBA", and a propagation coefficient is 3-4 in each subculture. KT had a strong inhibiting effect on the germination and growth of the axillary buds of Hibiscus esculentus L., and a strong dedifferentiating effect on the stem segments. About 25% of the calli, cultured on the medium"MS+0 5-1 0?mg/L IBA" for 50-60 days,transformed into somatic embryoids, which were visible to the naked eye. Most of the somatic embryoids will develope into the intact plantlets on the same medium 30 days later.
