为有效防控云南茶树重要的食叶性害虫茶谷蛾(Agriophara rhombata),筛选防治茶谷蛾的适用药及适用量,指导科学用药。在室内采用浸叶法测定24~96 h 4种不同稀释倍数杀虫剂对茶谷蛾3龄幼虫的毒力作用。结果表明,4种化学杀虫剂对3龄茶谷蛾...为有效防控云南茶树重要的食叶性害虫茶谷蛾(Agriophara rhombata),筛选防治茶谷蛾的适用药及适用量,指导科学用药。在室内采用浸叶法测定24~96 h 4种不同稀释倍数杀虫剂对茶谷蛾3龄幼虫的毒力作用。结果表明,4种化学杀虫剂对3龄茶谷蛾幼虫的毒力作用由强到弱表现为:24~72 h时,6%乙基多杀菌素悬浮剂(SC)>15%茚虫威乳油(EC)>15%唑虫酰胺SC>24%虫螨腈SC;96 h时,6%乙基多杀菌素SC>24%虫螨腈SC>15%茚虫威EC>15%唑虫酰胺SC。6%乙基多杀菌素SC与15%茚虫威EC具有较强毒杀力及速效性,可作为应急防治茶谷蛾的推荐药剂;24%虫螨腈SC发挥作用较缓,具有明显的时间—剂量—死亡率效应。研究结果可为云南茶区茶谷蛾的防治提供参考基础和依据。展开更多
[Objective] This study aimed to explore the biological habits of Scirothrips dorsalis Hood and methods for controlling this pest. [Method] From 2009 to 2011, the biological habits of Scirothrips dorsalis Hood and the ...[Objective] This study aimed to explore the biological habits of Scirothrips dorsalis Hood and methods for controlling this pest. [Method] From 2009 to 2011, the biological habits of Scirothrips dorsalis Hood and the regularity of its occurrence on mangoes were investigated at the experimental base of the Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences and Shuanghongqiao Mango Orchard in Baoshan, Yunnan Province. In addition, a field trial on the effects of four medicaments on controlling this pest was also conducted. [Result] The result showed that Scirothrips dorsalis Hood completed 10-12 genera- tions per year in Baoshan area, and the peak period of its incidence was from February to April. The results of the pest control testing showed that at 0.05 level, there were significant differences of effect on pest controlling among the treatments I, II and IV, and the treatment III showed significant differences of controlling effect with the treatment IV, while no significant differences with either the treatment I or the treatment II; at 0.01 level, there was no significant difference among the treat- ments I, II and III, while all the three treatments showed significant differences with treatment IV. [Conclusion] This study revealed that the occurrence and the severity of Scirothrips dorsalis Hood on mangoes in Baoshan, Yunnan Province. In addition, pesticides with certain efficacy on controlling Scirothrips dorsalis Hood were screened, which provides more options for fruit growers to control this pest on mangoes.展开更多
利用RT—PCR方法，自烟草丛顶病毒（Tobacco bush top virus，TBTV）龙陵分离物（TBTV—YLLi）的RNA中扩增出ORF3目的片段，并克隆到pMD18-T载体上进行序列分析．结果表明，TBTV-YLLi的ORF3全长714bp，与TBTV其他分离物的核苷酸相似性...利用RT—PCR方法，自烟草丛顶病毒（Tobacco bush top virus，TBTV）龙陵分离物（TBTV—YLLi）的RNA中扩增出ORF3目的片段，并克隆到pMD18-T载体上进行序列分析．结果表明，TBTV-YLLi的ORF3全长714bp，与TBTV其他分离物的核苷酸相似性和氨基酸相似性分别为98．3％～98．6％和95．4％～95．8％；与同属的花生丛簇病毒（Groundnut rosette virus，GRV）、豌豆耳突花叶病毒2号（Pea enation mosaic virus-2，PEMV-2）和胡萝卜拟斑驳病毒（Carrot mottle mimic virus，CMoMV）的核苷酸相似性分别为65．1％、61．6％和49．7％，氨基酸相似性分别为36．4％、34．6％和16．5％．将ORF3克隆到原核表达载体pET28a（＋）上，获得的重组子pET28-ORF3转化大肠杆菌BL21-plysS，使用终浓度为1．0mmol／L的IPTG在37℃诱导培养4h时，该融合蛋白表达量最高．融合蛋白经Ni^2＋亲和柱层析纯化后，SDS—PAGE电泳表明，融合蛋白相对分子质量约为35000，与预计的相对分子质量大小相一致．展开更多
基金Supported by the Special Fund for the Industrial Science and Technology Construction of Department of Agriculture(201202092-6)~~
文摘[Objective] This study aimed to explore the biological habits of Scirothrips dorsalis Hood and methods for controlling this pest. [Method] From 2009 to 2011, the biological habits of Scirothrips dorsalis Hood and the regularity of its occurrence on mangoes were investigated at the experimental base of the Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences and Shuanghongqiao Mango Orchard in Baoshan, Yunnan Province. In addition, a field trial on the effects of four medicaments on controlling this pest was also conducted. [Result] The result showed that Scirothrips dorsalis Hood completed 10-12 genera- tions per year in Baoshan area, and the peak period of its incidence was from February to April. The results of the pest control testing showed that at 0.05 level, there were significant differences of effect on pest controlling among the treatments I, II and IV, and the treatment III showed significant differences of controlling effect with the treatment IV, while no significant differences with either the treatment I or the treatment II; at 0.01 level, there was no significant difference among the treat- ments I, II and III, while all the three treatments showed significant differences with treatment IV. [Conclusion] This study revealed that the occurrence and the severity of Scirothrips dorsalis Hood on mangoes in Baoshan, Yunnan Province. In addition, pesticides with certain efficacy on controlling Scirothrips dorsalis Hood were screened, which provides more options for fruit growers to control this pest on mangoes.
