A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidylox...A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]展开更多
A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl...A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.展开更多
文摘A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]
文摘A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.
