The steady state of mRNAs for the α-and βA-subunits of inhibin was investigated in human corpora lutea(CL) during the menstrual cycle by Northern blot analysis with more sensitive cRNA probes. It revealed α 1.6kb m...The steady state of mRNAs for the α-and βA-subunits of inhibin was investigated in human corpora lutea(CL) during the menstrual cycle by Northern blot analysis with more sensitive cRNA probes. It revealed α 1.6kb mRNA for a subunit and 6.0,4.0, 2.8 and 1.8kb transcripts for βA subunit, Both of α-and βA inhibin mRNAs were detectable in CLs during early, mid and late luteal phases, but not in retrogressive CL after day-27 of the cycle or in tissues of ovarian stroma. An overall profile of展开更多
“——Few if any drugs with luteofytic unlion have proven to be of comraceptive value which may be due at teast in part,to our incomplete knowledge of the factors that conlrol the function and lifespan of the corpuslu...“——Few if any drugs with luteofytic unlion have proven to be of comraceptive value which may be due at teast in part,to our incomplete knowledge of the factors that conlrol the function and lifespan of the corpuslutenm——”展开更多
The structures of porcine, and ovine hypothalmic gonadotropin releasinghormone(GnRH) have been found to be identical It is a linear decapetide withdistinctive structural features and blocked N-and C-termini. Variants ...The structures of porcine, and ovine hypothalmic gonadotropin releasinghormone(GnRH) have been found to be identical It is a linear decapetide withdistinctive structural features and blocked N-and C-termini. Variants of GnRHare present in lower vertebrates (Table).展开更多
Previous work from our laboratory has demonstrated that T lymphocyte-derived cytokine,interferon-gamma(IFN-γ) may play a role in human luteal regression by inhibiting luteal progesterone production.Prostaglandin F2α...Previous work from our laboratory has demonstrated that T lymphocyte-derived cytokine,interferon-gamma(IFN-γ) may play a role in human luteal regression by inhibiting luteal progesterone production.Prostaglandin F2αhas been known as an important luteolytic factor in a wide range of mammalian species.It was of interest to investigate the effects of IFN-γon prostaglandin synthesis and their possible interaction with the inhibition on human luteal steroidogenesis.Human luteal cells were cultured for four days in the presence or absence of IFN-γ.Simultaneously, the productions of progesterone,prostaglandin F2α(PGF2α),Prostaglandin E2(PGE2),and 6-ketoprostaglandin F1α(PGF1α) were evaluated.Concomitant with the inhibition of progesterone production induced by IFN-γ,αbiphasic pattern of response of prostaglandin synthesis was observed,i.e.a slight decrease of PGF2αand PGF1αafterα48 h exposure to IFN-γ while an increase of PGE2 after 96 h. In a separate experiment,a luteotropic action of PGE2 and PGF2a on human luteal cells from different stages was observed during 48 and 96 h periods of culture.In addition,while indomethacin(INDO) treatment markedly blocked the prostaglandin synthesis, the hasal as well as hCG stimulated progesterone production was still inhibited by IFN-γas usual.These results suggested that prostaglandins appeared to be not responsible for the observed inhibition Of progesterone production since the inhibitory effect was not influenced by concurrent treatment with INDO which suppressed prostaglandin synthesis.展开更多
We should like to communicate,as a result of our combined efforts for the past five years,this preliminary report on the total synthesis of crystalline bovine insulin,the structure of which was first elucidated by San...We should like to communicate,as a result of our combined efforts for the past five years,this preliminary report on the total synthesis of crystalline bovine insulin,the structure of which was first elucidated by Sanger and coworkers[1]ten years ago.A number of papers on the synthetic work concerning insulin has been published during the past two years.Katsoyannis and coworkers[2,3].synthesized both the A and B chains of sheep insulin and,by combining them together according to the procedure of Dixon and Wardlow[4],observed only traces of insulin activity.Zahn,Meienhofer and co-展开更多
文摘The steady state of mRNAs for the α-and βA-subunits of inhibin was investigated in human corpora lutea(CL) during the menstrual cycle by Northern blot analysis with more sensitive cRNA probes. It revealed α 1.6kb mRNA for a subunit and 6.0,4.0, 2.8 and 1.8kb transcripts for βA subunit, Both of α-and βA inhibin mRNAs were detectable in CLs during early, mid and late luteal phases, but not in retrogressive CL after day-27 of the cycle or in tissues of ovarian stroma. An overall profile of
文摘“——Few if any drugs with luteofytic unlion have proven to be of comraceptive value which may be due at teast in part,to our incomplete knowledge of the factors that conlrol the function and lifespan of the corpuslutenm——”
文摘The structures of porcine, and ovine hypothalmic gonadotropin releasinghormone(GnRH) have been found to be identical It is a linear decapetide withdistinctive structural features and blocked N-and C-termini. Variants of GnRHare present in lower vertebrates (Table).
文摘Previous work from our laboratory has demonstrated that T lymphocyte-derived cytokine,interferon-gamma(IFN-γ) may play a role in human luteal regression by inhibiting luteal progesterone production.Prostaglandin F2αhas been known as an important luteolytic factor in a wide range of mammalian species.It was of interest to investigate the effects of IFN-γon prostaglandin synthesis and their possible interaction with the inhibition on human luteal steroidogenesis.Human luteal cells were cultured for four days in the presence or absence of IFN-γ.Simultaneously, the productions of progesterone,prostaglandin F2α(PGF2α),Prostaglandin E2(PGE2),and 6-ketoprostaglandin F1α(PGF1α) were evaluated.Concomitant with the inhibition of progesterone production induced by IFN-γ,αbiphasic pattern of response of prostaglandin synthesis was observed,i.e.a slight decrease of PGF2αand PGF1αafterα48 h exposure to IFN-γ while an increase of PGE2 after 96 h. In a separate experiment,a luteotropic action of PGE2 and PGF2a on human luteal cells from different stages was observed during 48 and 96 h periods of culture.In addition,while indomethacin(INDO) treatment markedly blocked the prostaglandin synthesis, the hasal as well as hCG stimulated progesterone production was still inhibited by IFN-γas usual.These results suggested that prostaglandins appeared to be not responsible for the observed inhibition Of progesterone production since the inhibitory effect was not influenced by concurrent treatment with INDO which suppressed prostaglandin synthesis.
文摘We should like to communicate,as a result of our combined efforts for the past five years,this preliminary report on the total synthesis of crystalline bovine insulin,the structure of which was first elucidated by Sanger and coworkers[1]ten years ago.A number of papers on the synthetic work concerning insulin has been published during the past two years.Katsoyannis and coworkers[2,3].synthesized both the A and B chains of sheep insulin and,by combining them together according to the procedure of Dixon and Wardlow[4],observed only traces of insulin activity.Zahn,Meienhofer and co-
