The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were ampl...The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were amplified, of which 135 polymorphic bands were discovered and the percentage of polymorphic bands （PPB） was 80.36%. A genetic diversity was revealed as Nei＇s gene diversity （H） = 0.2666 and Shannon information index （I） = 0.4028 at population level. The 34 populations were divided into different groups based on administrative regions, latitude and longitudes, river areas, altitudes of their origins, and their indexes such as Na （number of alleles）, Ne （effective number of alleles）, H, I and PPB were calculated. Richer genetic diversity was found in the wild rice populations distributed in Simao Prefecture than that in Lingcang Prefecture or Xishuangbanna Prefecture whereas the least genetic diversity was in Baoshan Prefecture or Dehong Prefecture. Rich genetic diversity was also discovered in the wild rice populations originated from higher than 710 m altitude around the middle and lower reaches of the Lancang River belonging to the Pacific Ocean drainage system. The 34 populations could be classified into two groups, one group covered the wild rice distributing in Simao Prefecture only while the other group covered ones in Lingcang, Xishuangbanna and Dehong Prefectures. The issue on how to effectively conserve the wild rice germplasm was discussed.展开更多
By using the parents, Kunmingxiaobaigu, a landrace variety with cold tolerance from Yunnan, China, and Towada, a cold-sensitive variety bred in Japan, as well as their F8 and F9 RIL progenies as test materials, cold t...By using the parents, Kunmingxiaobaigu, a landrace variety with cold tolerance from Yunnan, China, and Towada, a cold-sensitive variety bred in Japan, as well as their F8 and F9 RIL progenies as test materials, cold tolerance of rice at the booting stage in 2002 and 2003 under four different altitudes （environments）, and the correlationship between spikelet fertility and specific spikelet fertility （spikelet fertility of nine spikelets from the 3rd to the 5th spikelets of three primary branches at the top of each panicle） of single plant were analyzed, The data revealed a significant correlationship between spikelet fertility and specific spikelet fertility with the range from 0.7364 to 0.9011. However, under the cold stress environments, the correlation coefficients were higher than those without cold stress. On the other hand, the panicle exsertion was correlated to both spikelet fertility and specific spikelet fertility. Moreover, the heredity analysis suggested that specific spikelet fertility could replace spikelet fertility as an identification indicator, while panicle exsertion could be used as a partial indicator of cold tolerance identification at the booting stage in rice.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant No.30460065)Yunnan Provincial Foundation for Science.
文摘The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were amplified, of which 135 polymorphic bands were discovered and the percentage of polymorphic bands （PPB） was 80.36%. A genetic diversity was revealed as Nei＇s gene diversity （H） = 0.2666 and Shannon information index （I） = 0.4028 at population level. The 34 populations were divided into different groups based on administrative regions, latitude and longitudes, river areas, altitudes of their origins, and their indexes such as Na （number of alleles）, Ne （effective number of alleles）, H, I and PPB were calculated. Richer genetic diversity was found in the wild rice populations distributed in Simao Prefecture than that in Lingcang Prefecture or Xishuangbanna Prefecture whereas the least genetic diversity was in Baoshan Prefecture or Dehong Prefecture. Rich genetic diversity was also discovered in the wild rice populations originated from higher than 710 m altitude around the middle and lower reaches of the Lancang River belonging to the Pacific Ocean drainage system. The 34 populations could be classified into two groups, one group covered the wild rice distributing in Simao Prefecture only while the other group covered ones in Lingcang, Xishuangbanna and Dehong Prefectures. The issue on how to effectively conserve the wild rice germplasm was discussed.
基金The authors are thankful to Prof,ZHANG Hong-sheng from Nanjing Agricultural University for his kind suggestion on the manuscript.This study was supported by Yunnan Province Natural Science Foundation(No.2000C0093M)Program of introducing International Advanced Agro-technology,Ministry of Agriculture,China+1 种基金Hi-Tech Research and Development Program of Chinathe Key Technology Research and Development Program of the National Tenth Five-Year Plan(No.2004BA525B0207)of Ministry of Agriculture,China.
文摘By using the parents, Kunmingxiaobaigu, a landrace variety with cold tolerance from Yunnan, China, and Towada, a cold-sensitive variety bred in Japan, as well as their F8 and F9 RIL progenies as test materials, cold tolerance of rice at the booting stage in 2002 and 2003 under four different altitudes （environments）, and the correlationship between spikelet fertility and specific spikelet fertility （spikelet fertility of nine spikelets from the 3rd to the 5th spikelets of three primary branches at the top of each panicle） of single plant were analyzed, The data revealed a significant correlationship between spikelet fertility and specific spikelet fertility with the range from 0.7364 to 0.9011. However, under the cold stress environments, the correlation coefficients were higher than those without cold stress. On the other hand, the panicle exsertion was correlated to both spikelet fertility and specific spikelet fertility. Moreover, the heredity analysis suggested that specific spikelet fertility could replace spikelet fertility as an identification indicator, while panicle exsertion could be used as a partial indicator of cold tolerance identification at the booting stage in rice.
