The primary function of mitochondria is respiration, where catabolism of substrates is coupled to ATP synthesis via oxidative phosphorylation. In plants, mitochondrial composition is relatively complex and flexible an...The primary function of mitochondria is respiration, where catabolism of substrates is coupled to ATP synthesis via oxidative phosphorylation. In plants, mitochondrial composition is relatively complex and flexible and has specific pathways to support photosynthetic processes in illuminated leaves.展开更多
At12Cys-1 （At5g64400） and At12Cys-2 （At5g09570） are two closely related isogenes that encode small, twin cysteine proteins, typically located in mitochondria. At12Cys-2 transcript is induced in a variety of mutant...At12Cys-1 （At5g64400） and At12Cys-2 （At5g09570） are two closely related isogenes that encode small, twin cysteine proteins, typically located in mitochondria. At12Cys-2 transcript is induced in a variety of mutants with disrupted mitochondrial proteins, but an increase in At12Cys protein is only detected in mutants with reduced mitochondrial complex I abundance. Induction of At12Cys protein in mutants that lack mitochondrial complex I is accompanied by At12Cys protein located in mitochondria, chloroplasts, and the cytosoh Biochemical analyses revealed that even single gene deletions, i.e., At12cys-1 orAtl2cys-2, have an effect on mitochondrial and chloroplast functions. However, only double mutants, i.e., At12cys-1：At12cys.2, affect the abundance of protein and mRNA transcripts encoding translation elongation factors as well as rRNA abundance. Blue native PAGE showed that At12Cys co-migrated with mitochondrial supercomplex I ＋ lU. Likewise, deletion of both At12cys-1 and At12cys-2 genes, but not single gene deletions, results in enhanced tolerance to drought and light stress and increased anti-oxidant capacity. The induction and multiple localization of At12Cys upon a reduction in complex I abundance provides a mechanism to specifically signal mitochondrial dysfunction to the cytosol and then beyond to other organelles in the cell.展开更多
基金supported by the Australian Research Council (ARC) ARC Centre of Excellence for Plant Energy Biology(CE0561495)RPJ is supported by a Grains Research and Development Corporation (GRDC) PhD scholarship+1 种基金LL was funded by Scholarship International Research Fees (SIRF),University International Stipend (UIS) and a Top Up Scholarship for UISAHM is supported by the Australian Research Council(ARC) as an ARC Future Fellow
文摘The primary function of mitochondria is respiration, where catabolism of substrates is coupled to ATP synthesis via oxidative phosphorylation. In plants, mitochondrial composition is relatively complex and flexible and has specific pathways to support photosynthetic processes in illuminated leaves.
文摘At12Cys-1 （At5g64400） and At12Cys-2 （At5g09570） are two closely related isogenes that encode small, twin cysteine proteins, typically located in mitochondria. At12Cys-2 transcript is induced in a variety of mutants with disrupted mitochondrial proteins, but an increase in At12Cys protein is only detected in mutants with reduced mitochondrial complex I abundance. Induction of At12Cys protein in mutants that lack mitochondrial complex I is accompanied by At12Cys protein located in mitochondria, chloroplasts, and the cytosoh Biochemical analyses revealed that even single gene deletions, i.e., At12cys-1 orAtl2cys-2, have an effect on mitochondrial and chloroplast functions. However, only double mutants, i.e., At12cys-1：At12cys.2, affect the abundance of protein and mRNA transcripts encoding translation elongation factors as well as rRNA abundance. Blue native PAGE showed that At12Cys co-migrated with mitochondrial supercomplex I ＋ lU. Likewise, deletion of both At12cys-1 and At12cys-2 genes, but not single gene deletions, results in enhanced tolerance to drought and light stress and increased anti-oxidant capacity. The induction and multiple localization of At12Cys upon a reduction in complex I abundance provides a mechanism to specifically signal mitochondrial dysfunction to the cytosol and then beyond to other organelles in the cell.
