Restriction fragment length polymorphism （RFLP） profiles of the amplified products of Internal Transcribed Spacer （ITS） region of rDNA using four restriction enzymes （Alul, Rsal, HinfI and HhaI） revealed distinc...Restriction fragment length polymorphism （RFLP） profiles of the amplified products of Internal Transcribed Spacer （ITS） region of rDNA using four restriction enzymes （Alul, Rsal, HinfI and HhaI） revealed distinctness of six Indian isolates of Steinernema one each from Maharashtra （IARI-EPN-mh）, Himachal Pradesh （IARI-EPN-hp）, Dehradun （IARI-EPN-dhdl）, Jharkhand （IARI-EPN-jhl） and two from Madhya Pradesh （IARI-EPN-bpll ＆ IARI-EPN-gwll）, when compared with the only native species Steinernema thermophilum. One of the restriction enzyme, Rsal could differentiate all the six species/strains from one another. The three restriction enzymes yielded patterns which were of diagnostic value but Rsal appeared to be the best diagnostic marker for differentiating these isolates. A tree constructed based upon the band sharing amongst the isolates, produced trichotomy which placed strains from Madhya Pradesh and Jharkhand in one group showing 94% homology, one strain from Bhopal （M.P） formed separate clade along with S. thermophilum with 72% similarity. These isolates, from Maharashtra, Himachal Pradesh and Dehradun, showed only 51% similarity with the S. thermophilum by forming separate clade.展开更多
Vermicomposting is a biotechnological process that enables the recycling of organic waste materials into manure through the combined action of earthworms and mesophilic microorganisms. In this study, a 13-week experim...Vermicomposting is a biotechnological process that enables the recycling of organic waste materials into manure through the combined action of earthworms and mesophilic microorganisms. In this study, a 13-week experiment was carried to vermiprocess food industry sludge mixed with different bedding materials including two weeds, water hyacinth and parthenium, as well as cow dung, in different combinations employing earthworms of the species Eisenia fetida. Eight vermibins containing one kilogram of the waste mixtures （dry weight basis） were established for vermicomposting. Vermiprocessing significantly increased nitrogen, phosphorous, and potassium contents of the mixtures. However, a decrease in pH, organic carbon, and C：N ratio was observed after vermiprocessing. The heavy metal contents in the vermicomposts were higher than the initial values but within permissible limits. These results indicated that the studied wastes can be converted into good quality manure by vermiprocessing, which indicated their agricultural values as a soil conditioner if mixed with weeds in appropriate ratios.展开更多
文摘Restriction fragment length polymorphism （RFLP） profiles of the amplified products of Internal Transcribed Spacer （ITS） region of rDNA using four restriction enzymes （Alul, Rsal, HinfI and HhaI） revealed distinctness of six Indian isolates of Steinernema one each from Maharashtra （IARI-EPN-mh）, Himachal Pradesh （IARI-EPN-hp）, Dehradun （IARI-EPN-dhdl）, Jharkhand （IARI-EPN-jhl） and two from Madhya Pradesh （IARI-EPN-bpll ＆ IARI-EPN-gwll）, when compared with the only native species Steinernema thermophilum. One of the restriction enzyme, Rsal could differentiate all the six species/strains from one another. The three restriction enzymes yielded patterns which were of diagnostic value but Rsal appeared to be the best diagnostic marker for differentiating these isolates. A tree constructed based upon the band sharing amongst the isolates, produced trichotomy which placed strains from Madhya Pradesh and Jharkhand in one group showing 94% homology, one strain from Bhopal （M.P） formed separate clade along with S. thermophilum with 72% similarity. These isolates, from Maharashtra, Himachal Pradesh and Dehradun, showed only 51% similarity with the S. thermophilum by forming separate clade.
基金Supported by the University Grants Commission(UGC),India
文摘Vermicomposting is a biotechnological process that enables the recycling of organic waste materials into manure through the combined action of earthworms and mesophilic microorganisms. In this study, a 13-week experiment was carried to vermiprocess food industry sludge mixed with different bedding materials including two weeds, water hyacinth and parthenium, as well as cow dung, in different combinations employing earthworms of the species Eisenia fetida. Eight vermibins containing one kilogram of the waste mixtures （dry weight basis） were established for vermicomposting. Vermiprocessing significantly increased nitrogen, phosphorous, and potassium contents of the mixtures. However, a decrease in pH, organic carbon, and C：N ratio was observed after vermiprocessing. The heavy metal contents in the vermicomposts were higher than the initial values but within permissible limits. These results indicated that the studied wastes can be converted into good quality manure by vermiprocessing, which indicated their agricultural values as a soil conditioner if mixed with weeds in appropriate ratios.
