The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previous...The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previously demonstrated ultrahigh sensitivity to rare cells.This sensitivity originates from the minimal magnetic background in blood,obviating cumbersome and detrimental sample preparation.However,the translation of this technology to clinical applications has been limited by inherently low throughput(<1 mL/h),susceptibility to clogging,and incompatibility with commercial CMOS foundry processing.To help overcome these challenges,we have developed CMOS-compatible graphene Hall sensors for integration with PDMS microfluidics for magnetic sensing in blood.We demonstrate that these grapheneμHDs can match the performance of the best publishedμHDs,can be passivated for robust use with whole blood,and can be integrated with microfluidics and sensing electronics for in-flow detection of magnetic beads.We show a proof-of-concept validation of our system on a silicon substrate and detect magnetic agarose beads,as a model for cells,demonstrating promise for future integration in clinical applications with a custom CMOS chip.展开更多
基金This work was carried out in part at the Singh Center for Nanotechnology,which is supported by the NSF National Nanotechnology Coordinated Infrastructure Program under grant NNCI-2025608The authors would like to acknowledge support from National Institute of Allergy and Infectious Diseases(NIAID),R61AI147406from the National Cancer Institute(NCI),R33CA206907.We thank Chengyu Wen for help with the graphene mobility measurements.
文摘The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previously demonstrated ultrahigh sensitivity to rare cells.This sensitivity originates from the minimal magnetic background in blood,obviating cumbersome and detrimental sample preparation.However,the translation of this technology to clinical applications has been limited by inherently low throughput(<1 mL/h),susceptibility to clogging,and incompatibility with commercial CMOS foundry processing.To help overcome these challenges,we have developed CMOS-compatible graphene Hall sensors for integration with PDMS microfluidics for magnetic sensing in blood.We demonstrate that these grapheneμHDs can match the performance of the best publishedμHDs,can be passivated for robust use with whole blood,and can be integrated with microfluidics and sensing electronics for in-flow detection of magnetic beads.We show a proof-of-concept validation of our system on a silicon substrate and detect magnetic agarose beads,as a model for cells,demonstrating promise for future integration in clinical applications with a custom CMOS chip.
