The study was carried out to induce variations and stimulate callus induction, plant regeneration from different explants of two tomato （Lycopersicon esculentum Mill.） cultivars Trescantos and super Regina by using ...The study was carried out to induce variations and stimulate callus induction, plant regeneration from different explants of two tomato （Lycopersicon esculentum Mill.） cultivars Trescantos and super Regina by using tissue culture technique and Sodium azide as a chemical mutagens at concentrations （0.0, 2.0 and 4.0） mM under salinity stress condition at the levels（3.0, 6.0 and 9.0） dS/m. Different plant growth regulators were tested for their potentials in callus induction. The results revealed that treated seeds with SA （sodium azide） at concentration （2.0） mM increased seed germination percentage, seedling height and root length as compare to control treatment. While （4.0） mM concentration cause a reduction in all parameters mentioned above. Concerning to callus induction both cultivars showed a different response against different tested media with varying concentrations of plant growth regulators and despite their variable response to all tested media a combination of （2.0） mg from Kinetin （KIN） and lndol acetic acid （IAA） was found to be the most effective as compare to other treatments. Moreover, when callus transferred to a stressed media the variation was observed in explants fresh weight, and high reduction with the increment of salt level were recorded. Similarly the regeneration efficiency from stressed callus were observed at the level 3.0 and 6.0 dS/m while 9.0 dS/m the callus failed to regenerate plants for all three explants of both tomato cultivars.展开更多
文摘The study was carried out to induce variations and stimulate callus induction, plant regeneration from different explants of two tomato （Lycopersicon esculentum Mill.） cultivars Trescantos and super Regina by using tissue culture technique and Sodium azide as a chemical mutagens at concentrations （0.0, 2.0 and 4.0） mM under salinity stress condition at the levels（3.0, 6.0 and 9.0） dS/m. Different plant growth regulators were tested for their potentials in callus induction. The results revealed that treated seeds with SA （sodium azide） at concentration （2.0） mM increased seed germination percentage, seedling height and root length as compare to control treatment. While （4.0） mM concentration cause a reduction in all parameters mentioned above. Concerning to callus induction both cultivars showed a different response against different tested media with varying concentrations of plant growth regulators and despite their variable response to all tested media a combination of （2.0） mg from Kinetin （KIN） and lndol acetic acid （IAA） was found to be the most effective as compare to other treatments. Moreover, when callus transferred to a stressed media the variation was observed in explants fresh weight, and high reduction with the increment of salt level were recorded. Similarly the regeneration efficiency from stressed callus were observed at the level 3.0 and 6.0 dS/m while 9.0 dS/m the callus failed to regenerate plants for all three explants of both tomato cultivars.
