The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin(Cur)through nanoformulation with gum Arabic(GA)as a coating agent through an efficient synthetic approach.The antioxidant ...The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin(Cur)through nanoformulation with gum Arabic(GA)as a coating agent through an efficient synthetic approach.The antioxidant properties of the developed nanoparticles(Cur/GANPs)were assessed through several in vitro assays,such asβ-carotene bleaching activity,DPPH,and nitric oxide scavenging activities in addition to evaluating its inhibitory activity on angiotensinconverting enzyme(ACE).The cytotoxicity of Cur/GANPs was evaluated in vitro using different types of human cancer cells including breast cancer(MCF7,MDA-MB231),liver cancer(HepG2),and colon cancer(HT29)cells.The prepared particles displayed an elliptical shape with a size ranging between 20–260 nm and a potential difference of–15 mV.The Cur/GANPs exhibited significant antioxidant activity compared to free curcumin when using concentrations between 31.5 and 500μg/mL.The Cur/GANPs also had inhibited the growth of all cancer cell lines in a proportional trend with concentrations used.Hence,the encapsulation with gum Arabic has augmented the antioxidant and anti-neoplastic effects of Curcumin.Therefore,Cur/GANPs may have effective therapeutic properties in diseases attributed to oxidative stress like cancer and hypertension.展开更多
基金supported by the Malaysian Ministry of Higher Education Fundamental Research Grant Scheme(FRGS17-005-0571)Prototype Research Grant Scheme(PRGS19-005-0049)Ministry of Science,Technology,and Innovation(MOSTI)Grant No.SMF18-001-0001.
文摘The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin(Cur)through nanoformulation with gum Arabic(GA)as a coating agent through an efficient synthetic approach.The antioxidant properties of the developed nanoparticles(Cur/GANPs)were assessed through several in vitro assays,such asβ-carotene bleaching activity,DPPH,and nitric oxide scavenging activities in addition to evaluating its inhibitory activity on angiotensinconverting enzyme(ACE).The cytotoxicity of Cur/GANPs was evaluated in vitro using different types of human cancer cells including breast cancer(MCF7,MDA-MB231),liver cancer(HepG2),and colon cancer(HT29)cells.The prepared particles displayed an elliptical shape with a size ranging between 20–260 nm and a potential difference of–15 mV.The Cur/GANPs exhibited significant antioxidant activity compared to free curcumin when using concentrations between 31.5 and 500μg/mL.The Cur/GANPs also had inhibited the growth of all cancer cell lines in a proportional trend with concentrations used.Hence,the encapsulation with gum Arabic has augmented the antioxidant and anti-neoplastic effects of Curcumin.Therefore,Cur/GANPs may have effective therapeutic properties in diseases attributed to oxidative stress like cancer and hypertension.
