The structure of 1-hydroxy-3,5-dimethoxy-9 H-xanthen-9-one isolated from chloroform extract of Ajuga bracteosa root was analyzed by single-crystal X-ray diffraction. DPPH(1,1-diphenyl-2-picryl hydrazyl), ABTS(2,2?-azi...The structure of 1-hydroxy-3,5-dimethoxy-9 H-xanthen-9-one isolated from chloroform extract of Ajuga bracteosa root was analyzed by single-crystal X-ray diffraction. DPPH(1,1-diphenyl-2-picryl hydrazyl), ABTS(2,2?-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities(RSA) and Fe2+ chelating activities were carried out to determine the antioxidant potential of the compound. RSA values for the compound were 96%, 96% and 96% for all the three activities respectively at maximum concentration of the compound(100 μ gmL-1) with the IC50 values of 3.40, 4.86 and 0.10(μ gmL-1). Antidiabetic activities including antiglycation and α-glucosidase inhibition were also performed where the antiglycation activity was performed using two techniques including spectrofluorometric as well as spectrophotometric technique. Spectrofluorometric technique provided 97% antiglycation potential while 92% antiglycation potential was observed by spectrophotometric technique for the isolated compound. The compound at a concentration of 10 μ gmL-1 exhibited 31% α-glucosidase inhibitory potential with IC50 of 15.56 μ gmL-1. Antimicrobial activity data showed that the compound was active against all the studied pathogenic bacteria.展开更多
基金supported by Higher Education Commission(HEC)Govt.of Pakistan
文摘The structure of 1-hydroxy-3,5-dimethoxy-9 H-xanthen-9-one isolated from chloroform extract of Ajuga bracteosa root was analyzed by single-crystal X-ray diffraction. DPPH(1,1-diphenyl-2-picryl hydrazyl), ABTS(2,2?-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities(RSA) and Fe2+ chelating activities were carried out to determine the antioxidant potential of the compound. RSA values for the compound were 96%, 96% and 96% for all the three activities respectively at maximum concentration of the compound(100 μ gmL-1) with the IC50 values of 3.40, 4.86 and 0.10(μ gmL-1). Antidiabetic activities including antiglycation and α-glucosidase inhibition were also performed where the antiglycation activity was performed using two techniques including spectrofluorometric as well as spectrophotometric technique. Spectrofluorometric technique provided 97% antiglycation potential while 92% antiglycation potential was observed by spectrophotometric technique for the isolated compound. The compound at a concentration of 10 μ gmL-1 exhibited 31% α-glucosidase inhibitory potential with IC50 of 15.56 μ gmL-1. Antimicrobial activity data showed that the compound was active against all the studied pathogenic bacteria.
