Invertase(INV),a key enzyme in sucrose metabolism,irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose,thus playing important roles in plant growth,development,and biotic and abiotic stress respons...Invertase(INV),a key enzyme in sucrose metabolism,irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose,thus playing important roles in plant growth,development,and biotic and abiotic stress responses.In this study,we identified 27 members of the BnaINV family in Brassica napus.We constructed a phylogenetic tree of the family and predicted the gene structures,conserved motifs,cis-acting elements in promoters,physicochemical properties of encoded proteins,and chromosomal distribution of the BnaINVs.We also analyzed the expression of the BnaINVs in different tissues and developmental stages in the B.napus cultivar Zhongshuang 11 using qRT-PCR.In addition,we analyzed RNA-sequencing data to explore the expression patterns of the BnaINVs in four cultivars with different harvest indices and in plants inoculated with the pathogenic fungus Sclerotinia sclerotiorum.We used WGCNA(weighted coexpression network analysis)to uncover BnaINV regulatory networks.Finally,we explored the expression patterns of several BnaINV genes in cultivars with long(Zhongshuang 4)and short(Ningyou 12)siliques.Our results suggest that BnaINVs play important roles in the growth and development of rapeseed siliques and the defense response against pathogens.Our findings could facilitate the breeding of high-yielding B.napus cultivars with strong disease resistance.展开更多
目的:量化评价下颌全口义齿传统制作与数字化制作流程中各步骤对义齿基板与黏膜密合度的影响。方法:超硬石膏翻制6副下颌无牙颌标准模型,对同一石膏模型采用传统与数字化方法进行全口义齿基板制作。对传统制作流程中印模组织面,印模灌...目的:量化评价下颌全口义齿传统制作与数字化制作流程中各步骤对义齿基板与黏膜密合度的影响。方法:超硬石膏翻制6副下颌无牙颌标准模型,对同一石膏模型采用传统与数字化方法进行全口义齿基板制作。对传统制作流程中印模组织面,印模灌制石膏组织面,基板蜡型组织面以及注塑成型基托组织面进行三维扫描,在Geomagic studio 2014软件中进行偏差分析。对数字化制作流程中计算机辅助设计义齿基托组织面及3D打印基托组织面进行三维扫描与偏差分析。结果:传统全口义齿制作流程中,石膏灌制过程对基板与黏膜密合度影响为(170.50±10.63)μm,基托蜡型制作过程为(159.20±20.30)μm,基板注塑成型过程为(82.23±6.46)μm,印模制取过程为(61.13±7.75)μm。数字化制作流程中,基托打印成型过程产生偏差为(108.10±5.96)μm,CAD设计产生偏差为(98.46±6.18)μm。传统全口义齿制作方法成型基板与标准石膏模型组织面偏差为(165.10±12.73)μm,数字化方法为(125.06±6.59)μm。结论:数字化制作全口义齿基板与标准石膏模型组织面密合度优于传统制作全口义齿基板与标准石膏模型组织面密合度。传统方法偏差主要产生于石膏模型灌注与注塑两步,数字化方法偏差主要产生于基板的打印过程中。展开更多
基金This work was supported by grants from the National Natural Science Foundation of China(31830067)National Key Research and Development Program of China(2018YFD0100504-05)the“111”Project(B12006).
文摘Invertase(INV),a key enzyme in sucrose metabolism,irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose,thus playing important roles in plant growth,development,and biotic and abiotic stress responses.In this study,we identified 27 members of the BnaINV family in Brassica napus.We constructed a phylogenetic tree of the family and predicted the gene structures,conserved motifs,cis-acting elements in promoters,physicochemical properties of encoded proteins,and chromosomal distribution of the BnaINVs.We also analyzed the expression of the BnaINVs in different tissues and developmental stages in the B.napus cultivar Zhongshuang 11 using qRT-PCR.In addition,we analyzed RNA-sequencing data to explore the expression patterns of the BnaINVs in four cultivars with different harvest indices and in plants inoculated with the pathogenic fungus Sclerotinia sclerotiorum.We used WGCNA(weighted coexpression network analysis)to uncover BnaINV regulatory networks.Finally,we explored the expression patterns of several BnaINV genes in cultivars with long(Zhongshuang 4)and short(Ningyou 12)siliques.Our results suggest that BnaINVs play important roles in the growth and development of rapeseed siliques and the defense response against pathogens.Our findings could facilitate the breeding of high-yielding B.napus cultivars with strong disease resistance.
文摘目的:量化评价下颌全口义齿传统制作与数字化制作流程中各步骤对义齿基板与黏膜密合度的影响。方法:超硬石膏翻制6副下颌无牙颌标准模型,对同一石膏模型采用传统与数字化方法进行全口义齿基板制作。对传统制作流程中印模组织面,印模灌制石膏组织面,基板蜡型组织面以及注塑成型基托组织面进行三维扫描,在Geomagic studio 2014软件中进行偏差分析。对数字化制作流程中计算机辅助设计义齿基托组织面及3D打印基托组织面进行三维扫描与偏差分析。结果:传统全口义齿制作流程中,石膏灌制过程对基板与黏膜密合度影响为(170.50±10.63)μm,基托蜡型制作过程为(159.20±20.30)μm,基板注塑成型过程为(82.23±6.46)μm,印模制取过程为(61.13±7.75)μm。数字化制作流程中,基托打印成型过程产生偏差为(108.10±5.96)μm,CAD设计产生偏差为(98.46±6.18)μm。传统全口义齿制作方法成型基板与标准石膏模型组织面偏差为(165.10±12.73)μm,数字化方法为(125.06±6.59)μm。结论:数字化制作全口义齿基板与标准石膏模型组织面密合度优于传统制作全口义齿基板与标准石膏模型组织面密合度。传统方法偏差主要产生于石膏模型灌注与注塑两步,数字化方法偏差主要产生于基板的打印过程中。
