Mesenchymal stem cells(MSC)have pushed the field of stem cell-based therapies by inducing tissue regeneration,immunosuppression,and angiogenesis mainly through vesicles and soluble factors release(paracrine signaling)...Mesenchymal stem cells(MSC)have pushed the field of stem cell-based therapies by inducing tissue regeneration,immunosuppression,and angiogenesis mainly through vesicles and soluble factors release(paracrine signaling).MSC-extracellular vesicles(MSC-EV)adaptable secretome and homing to injured sites allowed researchers to unlock a new era of cell-free based therapy.In parallel,nanoparticles(NP)have been explored in contributing to transport and drug delivery systems,giving drugs desired physical-chemical properties to exploit cell behavior.However,NPs can be quickly recognized by immune cells and cleared from circulation.In this viewpoint,we explore how combining both therapeutic strategies can improve efficacy and circumvent limitations of both therapies.MSCEV benefit from the potent MSC membrane composition,guiding chemotaxis to tumor sites,a very restricted microenvironment.MSC-EV has low immunogenicity,high stability,long half-life and can explore tissue targeting ligands as a precise drug carry,even across biological barriers.Those properties promote enhanced targeted drug delivery that can be combined with NP,exploring biological membrane production through:1.direct cell therapy with NP-infused MSC;2.NP-containing MSC-EV generated by NP-infused MSC;3.by coating NP in MSC membrane(“MSC NanoGhosts”),allowing precise cargo definition without losing targeting.Therefore,nanotechnology combined with cell-based therapeutic resources can greatly improve targeted drug delivery,improving efficacy and opening a new venue of therapeutic possibilities.展开更多
基金This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil(CAPES)-Finance Code 001the“Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)”the“Fundação Carlos Chagas Filho de AmparoàPesquisa do Estado do Rio de Janeiro(FAPERJ)”.
文摘Mesenchymal stem cells(MSC)have pushed the field of stem cell-based therapies by inducing tissue regeneration,immunosuppression,and angiogenesis mainly through vesicles and soluble factors release(paracrine signaling).MSC-extracellular vesicles(MSC-EV)adaptable secretome and homing to injured sites allowed researchers to unlock a new era of cell-free based therapy.In parallel,nanoparticles(NP)have been explored in contributing to transport and drug delivery systems,giving drugs desired physical-chemical properties to exploit cell behavior.However,NPs can be quickly recognized by immune cells and cleared from circulation.In this viewpoint,we explore how combining both therapeutic strategies can improve efficacy and circumvent limitations of both therapies.MSCEV benefit from the potent MSC membrane composition,guiding chemotaxis to tumor sites,a very restricted microenvironment.MSC-EV has low immunogenicity,high stability,long half-life and can explore tissue targeting ligands as a precise drug carry,even across biological barriers.Those properties promote enhanced targeted drug delivery that can be combined with NP,exploring biological membrane production through:1.direct cell therapy with NP-infused MSC;2.NP-containing MSC-EV generated by NP-infused MSC;3.by coating NP in MSC membrane(“MSC NanoGhosts”),allowing precise cargo definition without losing targeting.Therefore,nanotechnology combined with cell-based therapeutic resources can greatly improve targeted drug delivery,improving efficacy and opening a new venue of therapeutic possibilities.
