8种品质成就一个好男人

当我们寻找＂真命天子＂时,先在脑海里想想他应该是什么样的总是没错的。不过,我可没在这里给女性传授什么恋爱真经,而是要从男人说起。我想想就觉得好笑,因为＂女人究竟想要从男人那里得到什么＂是我一直想要告诉他们的。在现实生活中,要找到具有这些品质的男士并不像多数乏善可陈的男人所认为的那样＂异想天开＂。

Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy

Amyloid proteins are associated with a broad spectrum of neurodegenerative diseases.However,it remains a grand challenge to extract molecular structure information from intracellular amyloid proteins in their native cellular environment.To address this challenge,we developed a computational chemical microscope integrating 3D midinfrared photothermal imaging with fluorescence imaging,termed Fluorescence-guided Bond-Selective Intensity Diffraction Tomography(FBS-IDT).Based on a low-cost and simple optical design,FBS-IDT enables chemical-specific volumetric imaging and 3D site-specific mid-IR fingerprint spectroscopic analysis of tau fbrils,an important type of amyloid protein aggregates,in their intracellular environment.Label-free volumetric chemical imaging of human cells with/without seeded tau fibrils is demonstrated to show the potential correlation between lipid accumulation and tau aggregate formation.Depth-resolved mid-infrared fingerprint spectroscopy is performed to reveal the protein secondary structure of the intracellular tau fibrils.3D visualization of theβ-sheet for tau fibril structure is achieved.

High-speed in vitro intensity diffraction tomography

We demonstrate a label-free,scan-free intensity diffraction tomography technique utilizing annular illumination(aIDT)to rapidly characterize large-volume three-dimensional(3-D)refractive index distributions in vitro.By optimally matching the illumination geometry to the microscope pupil,our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume rates.Using eight intensity images,we recover volumes of∼350μm×100μm×20μm,with near diffraction-limited lateral resolution of∼487 nm and axial resolution of∼3.4μm.The attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length scales.We demonstrate aIDT’s capabilities on unicellular diatom microalgae,epithelial buccal cell clusters with native bacteria,and live Caenorhabditis elegans specimens.Within these samples,we recover macroscale cellular structures,subcellular organelles,and dynamic micro-organism tissues with minimal motion artifacts.Quantifying such features has significant utility in oncology,immunology,and cellular pathophysiology,where these morphological features are evaluated for changes in the presence of disease,parasites,and new drug treatments.Finally,we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed technique.aIDT shows promise as a powerful high-speed,label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.

Resolution-enhanced intensity diffraction tomography in high numerical aperture label-free microscopy

We propose label-free and motion-free resolution-enhanced intensity diffraction tomography(reIDT)recovering the 3D complex refractive index distribution of an object.By combining an annular illumination strategy with a high numerical aperture(NA)condenser,we achieve near-diffraction-limited lateral resolution of 346 nm and axial resolution of 1.2μm over 130μm×130μm×8μm volume.Our annular pattern matches the system’s maximum NA to reduce the data requirement to 48 intensity frames.The reIDT system is directly built on a standard commercial microscope with a simple LED array source and condenser lens adds-on,and promises broad applications for natural biological imaging with minimal hardware modifications.To test the capabilities of our technique,we present the 3D complex refractive index reconstructions on an absorptive USAF resolution target and Henrietta Lacks(HeLa)and HT29 human cancer cells.Our work provides an important step in intensity-based diffraction tomography toward high-resolution imaging applications.