月影寿的组织培养与快速繁殖

选用月影寿Haworthia Tsukikage幼嫩花茎作为试验材料,研究不同消毒时间和灭菌处理对外植体的影响,结果表明,月影寿花茎的最佳灭菌处理为75%乙醇处理30s,0.1%HgCl2处理5～7min。应用正交试验设计研究基本培养基、植物生长调节剂配比及质量浓度水平变化对花茎愈伤组织诱导、分化和生根的影响,结果表明,最佳愈伤组织诱导培养基为:MS+2.0mg·L-1 6-BA+0.3mg·L-1 NAA+1.0mg·L-1 KT;最佳愈伤组织分化培养基为:1.0mg·L-1 6-BA+0.1mg·L-1 NAA+1.5mg·L-1 KT;最佳生根诱导培养基为1/2MS+0.1mg·L-1 NAA+5g·L-1活性炭,组培生根苗移栽成活率在85%以上。初步建立了月影寿组织培养与快速繁殖技术体系,为规模化生产提供了技术支持。

Ultrasound-promoted Lipase-catalyzed Enantioselective Transesterification of (R,S)-Glycidol

Enantioselective transesterification of glycidol with vinyl butyrate as an acyl donor was investigated in the presence of Bacillus subtilis lipase（BSL2） as catalyst. Comparison studies demonstrate the advantage of ultrasound over the conventional shaking for the enzymatic reaction in non-aqueous media. The effects of reaction conditions（ultrasound power, temperature, water activity and pH） on the activity and enantioselectivity were also investigated. Under the optimum conditions, the synthetic activity of BSL2 was 2.95 μmol？min？1？mg？1 and the enantioselectivity（E value） was 52.2. Compared with conventional shaking, ultrasound made the synthetic activity and the enantioselectivity increase 9.5-fold and 1.4-fold, respectively. Furthermore, the repeated use of BSL2 for five cycles resulted in no obvious loss of enzyme activity, suggesting that the enzyme is stable under low power ultrasound conditions.