Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the ...Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the eIF1A gene involved in fruit development and stress response of mango.In this study,the MieIF1A-b gene was isolated from Mangifera indica,and contains a 435-bp open reading frame,which encodes a putative protein of 144 amino acids(GenBank accession number:KP676599).The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6.Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis,Manihot esculenta,and Populus trichocarpa,with 96 and 95%identity,respectively.Quantitative reverse transcriptative PCR(qRT-PCR)analyses indicated that MieIF1A-b was expressed in all tested tissues,and had the highest expression level in fruit 80 d after flowering.The expression of MieIF1A-b was obviously regulated by NaCl and H2O2 treatments in leaves.Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth,phenotype and salinity tolerance compared with wild-type(WT)plants.The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation,and it was a candidate gene for abiotic stress in mango.展开更多
基金supported by the National Natural Science Foundation of China (31660561)the Natural Science Foundation of Guangxi, China (2015GXNSFAA139052)+3 种基金the Key Research and Development Project of Guangxi, China (GXKJ-AB17292010)the Major Science and Technology Projects of Guangxi, China (GXKJ-AA17204097-3 and GXKJ-AA172040 26-2)the Innovation Team of Guangxi Mango Industry Project, China (nycytxgxcxtd-06-02)the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China (SKLCUSA-a201906)
文摘Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the eIF1A gene involved in fruit development and stress response of mango.In this study,the MieIF1A-b gene was isolated from Mangifera indica,and contains a 435-bp open reading frame,which encodes a putative protein of 144 amino acids(GenBank accession number:KP676599).The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6.Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis,Manihot esculenta,and Populus trichocarpa,with 96 and 95%identity,respectively.Quantitative reverse transcriptative PCR(qRT-PCR)analyses indicated that MieIF1A-b was expressed in all tested tissues,and had the highest expression level in fruit 80 d after flowering.The expression of MieIF1A-b was obviously regulated by NaCl and H2O2 treatments in leaves.Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth,phenotype and salinity tolerance compared with wild-type(WT)plants.The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation,and it was a candidate gene for abiotic stress in mango.
