Male infertility has evolved from a common reproductive system disease to a major social issue.Youjing granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampnes...Male infertility has evolved from a common reproductive system disease to a major social issue.Youjing granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampness due to its pathogenic characteristics.YG has been shown to regulate sperm quality in clinical trials,but the underlying mechanism is not fully understood.The present study was aimed to explore the protective effects and mechanism of action of YG on male reproductive system damage caused by methyl methane sulfonate(MMS).We first established an infertility model of rats through oral administration of MMS and then treated with YG.To determine the effect of YG,spermatogenesis,microvascular density,and secretory function of Leydig cells and Sertoli cells in rats were assessed.Spermatogonial stem cells(SSCs)were co-cultured with mouse embryo fibroblast(MEF)cells as an in vitro cell model before exposure to serum containing YG.Furthermore,the proliferation and apoptosis of SSCs were measured.Results indicated that YG increased the expression of self-renewal and proliferation-related molecules such as glial cell line derived neurotrophic factor(GDNF)and fibroblast growth factor-2(FGF2),and improved the quality of sperm and the proliferation of SSCs.In conclusion,YG may protect spermatogenetic function of rats through regulating the proliferation and self-renewal of SSCs.展开更多
基金supported by the National Natural Science Foundation of China(No.81774314)the Foundation of Clinical Science and Technology of Wuxi(No.Q202059)+1 种基金the Top Talent Support Program for Young and Middle-Aged People of Wuxi Health Committee(Nos.BJ2020105 and HB2020108)the Clinical Medical Science and Technology Development Foundation of Jiangsu University(No.JYL20180068)。
文摘Male infertility has evolved from a common reproductive system disease to a major social issue.Youjing granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampness due to its pathogenic characteristics.YG has been shown to regulate sperm quality in clinical trials,but the underlying mechanism is not fully understood.The present study was aimed to explore the protective effects and mechanism of action of YG on male reproductive system damage caused by methyl methane sulfonate(MMS).We first established an infertility model of rats through oral administration of MMS and then treated with YG.To determine the effect of YG,spermatogenesis,microvascular density,and secretory function of Leydig cells and Sertoli cells in rats were assessed.Spermatogonial stem cells(SSCs)were co-cultured with mouse embryo fibroblast(MEF)cells as an in vitro cell model before exposure to serum containing YG.Furthermore,the proliferation and apoptosis of SSCs were measured.Results indicated that YG increased the expression of self-renewal and proliferation-related molecules such as glial cell line derived neurotrophic factor(GDNF)and fibroblast growth factor-2(FGF2),and improved the quality of sperm and the proliferation of SSCs.In conclusion,YG may protect spermatogenetic function of rats through regulating the proliferation and self-renewal of SSCs.
