AIM: To determine if hyperpolarisation-activated nucleotide-gated(HCN) channels exist in human colon, and to investigate the expression of HCN channels in Hirschsprung's disease.METHODS:We investigated HCN1,HCN2,H...AIM: To determine if hyperpolarisation-activated nucleotide-gated(HCN) channels exist in human colon, and to investigate the expression of HCN channels in Hirschsprung's disease.METHODS:We investigated HCN1,HCN2,HCN3 and HCN4 protein expression in pull-through specimens from patients with Hirschsprung’s disease(HSCR,n=10)using the proximal-most ganglionic segment and distalmost aganglionic segment,as well as in healthy control specimens obtained at the time of sigmoid colostomy closure in children who had undergone anorectoplasty for imperforate anus(n=10).Fluorescent immunohistochemistry was performed to assess protein distribution,which was then visualized using confocal microscopy.RESULTS:No HCN1 channel expression was observed in any of the tissues studied.Both HCN2 and HCN4proteins were found to be equally expressed in the aganglionic and ganglionic bowel in HSCR and controls.HCN3 channel expression was found to be markedly decreased in the aganglionic colon vs ganglionic colon and controls.HCN2-4 channels were seen to be expressed within neurons of the myenteric and submucosal plexus of the ganglionic bowel and normal controls,and also co-localised to interstitial cells of Cajal in all tissues studied.CONCLUSION:We demonstrate HCN channel expression in human colon for the first time.Reduced HCN3expression in aganglionic bowel suggests its potential role in HSCR pathophysiology.展开更多
Recently,the Sixth Charming Girl China Model Contest 2014 drew to close.This contest was held by China Costume Designer Association Costume Model Committee,and organized by China Bentley Culture Development Co.,Ltd.Al...Recently,the Sixth Charming Girl China Model Contest 2014 drew to close.This contest was held by China Costume Designer Association Costume Model Committee,and organized by China Bentley Culture Development Co.,Ltd.Almost ten thousand charming models attended the selection contest in 15 contest regions and after half a year 30 beauties went to the finals for top awards.Finally No.5展开更多
Lots of local fashion brands such as SEPTWOLVES,Ports,Joeone,and more than 50 international brands such as Louis Vuitton and Gucci,have opened shops in Xiamen.Xiamen hasdeveloped a fashion industry chain that encompas...Lots of local fashion brands such as SEPTWOLVES,Ports,Joeone,and more than 50 international brands such as Louis Vuitton and Gucci,have opened shops in Xiamen.Xiamen hasdeveloped a fashion industry chain that encompasses dresses,wedding dresses,coffee,tea,and fashion art;and it has also established a fashion district.Famous Chinese展开更多
On December 23,2014,the first exhibition of clothes designed by NETIGER for APEC leaders opened in Beijing.As the first stop in a series of national exhibitions,there were 40 sets of clothes on display,including men’...On December 23,2014,the first exhibition of clothes designed by NETIGER for APEC leaders opened in Beijing.As the first stop in a series of national exhibitions,there were 40 sets of clothes on display,including men’s and women’s clothes,and sleepwear.According to Mr.ZhangZhifeng,the founder and artistic director of NE·TIGER,the company contributed in展开更多
The 17th China Fashion Week made its grand debut in Beijing on October 25.As an opening show,NETIGER"Ming Li"Haute Couture Huafu Fashion Show was performed in a great way in Beijing Hotel.The Ritual and Musi...The 17th China Fashion Week made its grand debut in Beijing on October 25.As an opening show,NETIGER"Ming Li"Haute Couture Huafu Fashion Show was performed in a great way in Beijing Hotel.The Ritual and Music Civilization Built in a Country Famed for Its Clothing Culture On the stage,as the booming sound of violoncellos was integrated with the展开更多
On November 3,2014 China’s only"national gift"pearl brand"Ruans Jewelry"and China Bentley Culture Development Co.,Ltd orchestrated a gala ceremony that heralds the opening of the first internation...On November 3,2014 China’s only"national gift"pearl brand"Ruans Jewelry"and China Bentley Culture Development Co.,Ltd orchestrated a gala ceremony that heralds the opening of the first international flagship store of"Ruans Pearl"brand at Blue Harbor.According to Sun Fengmin,secretary of China Gems&Jewelry Trade Association,the opening of Rusans flagship store marks the new era of the jewelry industry development:the flagship is no more a single展开更多
During Mercedes-Benz China Fashion Week,QIAODAN Cup 9th China Sporting Equipment Design Contest was co-held by China Fashion Association and QIAODAN Sports Co.,Ltd in Beijing 751D-PARK workshop on October 28th.Meanwhi...During Mercedes-Benz China Fashion Week,QIAODAN Cup 9th China Sporting Equipment Design Contest was co-held by China Fashion Association and QIAODAN Sports Co.,Ltd in Beijing 751D-PARK workshop on October 28th.Meanwhile Qiaodan’s excellent designer Yin Peng presented the fashion trend of 2016 s/s with his"WEI"collection.Themed as"passion-applause",QIAODAN Cup 9th China Sporting Equipment Design Contest presents the enthusiasm and sincerity from Qiaodan sports for展开更多
On October 27,the new trading platform,DHUB Beijing made its debut on Tank Zone of 751 D·PARK and DANG·DHUB designer’s online platform was officially launched.DHUB Beijing extends the function of China Fash...On October 27,the new trading platform,DHUB Beijing made its debut on Tank Zone of 751 D·PARK and DANG·DHUB designer’s online platform was officially launched.DHUB Beijing extends the function of China Fashion Week,serving as a kind of role model in this industry.DHUB Beijing founded by展开更多
To mark the eleventh year of its sponsorship of the Beijing Music Festival,UBS sponsored a special concert by world-renowned Chinese pianist,YUNDI LI,at the Wangfujing Church in October.YUNDI’s performance at the ann...To mark the eleventh year of its sponsorship of the Beijing Music Festival,UBS sponsored a special concert by world-renowned Chinese pianist,YUNDI LI,at the Wangfujing Church in October.YUNDI’s performance at the annual Church Concert,which has been a feature of the Beijing Music Festival since its inception in 2006,marks his return to the Festival after a four-year absence.Taking place on展开更多
There are 230 million children under the age of 16 in China.According to the statistics from China Shanghai International Children’s Book Fair,children’s new book sales in China increased by 11%year on year and acco...There are 230 million children under the age of 16 in China.According to the statistics from China Shanghai International Children’s Book Fair,children’s new book sales in China increased by 11%year on year and accounted for 50%of the top 100 bestselling titles in the first six months of 2014.As the only one of its type covering the Asia-Pacific region,the second Shanghai International Children’s Book Fair(CCBF)is on course to be a stunning success."Children’s book sales in展开更多
欧内斯特·海明威(Ernest Hemingway,1899~1961),美国小说家。海明威被誉为美利坚民族的精神丰碑,是"新闻体"小说的创始人。其写作风格以简洁著称,对美国文学及20世纪文学的发展有极深远的影响。1953年,他凭借《老人与海...欧内斯特·海明威(Ernest Hemingway,1899~1961),美国小说家。海明威被誉为美利坚民族的精神丰碑,是"新闻体"小说的创始人。其写作风格以简洁著称,对美国文学及20世纪文学的发展有极深远的影响。1953年,他凭借《老人与海》获得普利策奖,1954年又获诺贝尔文学奖。《老人与海》(The Old Man and the Sea,1952)之外,海明威的其他代表作有:小说《太阳照常升起》(The Sun Also Rises,1926)、《永别了,武器》(A Farewell to Arms,1929).展开更多
CJ azit是一个为成长中的艺术家而建的剧场空间,坐落在韩国首尔的大学路上.这个极具工作室风格的建筑可以容纳包括音乐、戏剧、舞台表演等不同类型的演出.自2009年对外开放以来,它一直扮演着艺术孵化器的角色.前不久,CJ azit 由Betwin S...CJ azit是一个为成长中的艺术家而建的剧场空间,坐落在韩国首尔的大学路上.这个极具工作室风格的建筑可以容纳包括音乐、戏剧、舞台表演等不同类型的演出.自2009年对外开放以来,它一直扮演着艺术孵化器的角色.前不久,CJ azit 由Betwin Space Design接手,改建一新.展开更多
基金Supported by National Children’s Research Centre/Children’s Medical Research Foundation,Ireland
文摘AIM: To determine if hyperpolarisation-activated nucleotide-gated(HCN) channels exist in human colon, and to investigate the expression of HCN channels in Hirschsprung's disease.METHODS:We investigated HCN1,HCN2,HCN3 and HCN4 protein expression in pull-through specimens from patients with Hirschsprung’s disease(HSCR,n=10)using the proximal-most ganglionic segment and distalmost aganglionic segment,as well as in healthy control specimens obtained at the time of sigmoid colostomy closure in children who had undergone anorectoplasty for imperforate anus(n=10).Fluorescent immunohistochemistry was performed to assess protein distribution,which was then visualized using confocal microscopy.RESULTS:No HCN1 channel expression was observed in any of the tissues studied.Both HCN2 and HCN4proteins were found to be equally expressed in the aganglionic and ganglionic bowel in HSCR and controls.HCN3 channel expression was found to be markedly decreased in the aganglionic colon vs ganglionic colon and controls.HCN2-4 channels were seen to be expressed within neurons of the myenteric and submucosal plexus of the ganglionic bowel and normal controls,and also co-localised to interstitial cells of Cajal in all tissues studied.CONCLUSION:We demonstrate HCN channel expression in human colon for the first time.Reduced HCN3expression in aganglionic bowel suggests its potential role in HSCR pathophysiology.
文摘Recently,the Sixth Charming Girl China Model Contest 2014 drew to close.This contest was held by China Costume Designer Association Costume Model Committee,and organized by China Bentley Culture Development Co.,Ltd.Almost ten thousand charming models attended the selection contest in 15 contest regions and after half a year 30 beauties went to the finals for top awards.Finally No.5
文摘Lots of local fashion brands such as SEPTWOLVES,Ports,Joeone,and more than 50 international brands such as Louis Vuitton and Gucci,have opened shops in Xiamen.Xiamen hasdeveloped a fashion industry chain that encompasses dresses,wedding dresses,coffee,tea,and fashion art;and it has also established a fashion district.Famous Chinese
文摘On December 23,2014,the first exhibition of clothes designed by NETIGER for APEC leaders opened in Beijing.As the first stop in a series of national exhibitions,there were 40 sets of clothes on display,including men’s and women’s clothes,and sleepwear.According to Mr.ZhangZhifeng,the founder and artistic director of NE·TIGER,the company contributed in
文摘The 17th China Fashion Week made its grand debut in Beijing on October 25.As an opening show,NETIGER"Ming Li"Haute Couture Huafu Fashion Show was performed in a great way in Beijing Hotel.The Ritual and Music Civilization Built in a Country Famed for Its Clothing Culture On the stage,as the booming sound of violoncellos was integrated with the
文摘On November 3,2014 China’s only"national gift"pearl brand"Ruans Jewelry"and China Bentley Culture Development Co.,Ltd orchestrated a gala ceremony that heralds the opening of the first international flagship store of"Ruans Pearl"brand at Blue Harbor.According to Sun Fengmin,secretary of China Gems&Jewelry Trade Association,the opening of Rusans flagship store marks the new era of the jewelry industry development:the flagship is no more a single
文摘During Mercedes-Benz China Fashion Week,QIAODAN Cup 9th China Sporting Equipment Design Contest was co-held by China Fashion Association and QIAODAN Sports Co.,Ltd in Beijing 751D-PARK workshop on October 28th.Meanwhile Qiaodan’s excellent designer Yin Peng presented the fashion trend of 2016 s/s with his"WEI"collection.Themed as"passion-applause",QIAODAN Cup 9th China Sporting Equipment Design Contest presents the enthusiasm and sincerity from Qiaodan sports for
文摘On October 27,the new trading platform,DHUB Beijing made its debut on Tank Zone of 751 D·PARK and DANG·DHUB designer’s online platform was officially launched.DHUB Beijing extends the function of China Fashion Week,serving as a kind of role model in this industry.DHUB Beijing founded by
文摘To mark the eleventh year of its sponsorship of the Beijing Music Festival,UBS sponsored a special concert by world-renowned Chinese pianist,YUNDI LI,at the Wangfujing Church in October.YUNDI’s performance at the annual Church Concert,which has been a feature of the Beijing Music Festival since its inception in 2006,marks his return to the Festival after a four-year absence.Taking place on
文摘There are 230 million children under the age of 16 in China.According to the statistics from China Shanghai International Children’s Book Fair,children’s new book sales in China increased by 11%year on year and accounted for 50%of the top 100 bestselling titles in the first six months of 2014.As the only one of its type covering the Asia-Pacific region,the second Shanghai International Children’s Book Fair(CCBF)is on course to be a stunning success."Children’s book sales in
文摘欧内斯特·海明威(Ernest Hemingway,1899~1961),美国小说家。海明威被誉为美利坚民族的精神丰碑,是"新闻体"小说的创始人。其写作风格以简洁著称,对美国文学及20世纪文学的发展有极深远的影响。1953年,他凭借《老人与海》获得普利策奖,1954年又获诺贝尔文学奖。《老人与海》(The Old Man and the Sea,1952)之外,海明威的其他代表作有:小说《太阳照常升起》(The Sun Also Rises,1926)、《永别了,武器》(A Farewell to Arms,1929).
