The present study isolated and identified 32 species of endophytic mycobiota belonging to 18 genera associated with 8 wild medicinal plants collected from El-Kharga Oasis,New Valley Governorate,Egypt.Fusarium was the ...The present study isolated and identified 32 species of endophytic mycobiota belonging to 18 genera associated with 8 wild medicinal plants collected from El-Kharga Oasis,New Valley Governorate,Egypt.Fusarium was the most common genus followed by Alternaria and Aspergillus.Convolvulus arvensis was the plant with the highest number of endophytes over the other plant species,while Moringa oleifera reported the lowest number of endophytes.In addition,the entomopathogenic fungus Beauveria bassiana;was recorded for the first time from leaves of Portulaca oleracea.One hundred and twenty-three isolates representing 32 species were screened for their abilities to produce pectinase,carboxy methyl cellulase(CMCase)and avicellase enzymes on sucrose free-Cz supplemented,individually with 1%pectin or 1%CMC or 1%avicel as a sole carbon source,respectively.Ninety-four isolates produced pectinase while 66 isolates produced cellulases.The quantitative assays of the three enzymes for high-producers were performed in submerged fermentation using sucrose-free Cz broth.Aspergillus was the superior in the production of the three enzymes with the potent strains were A.terreus AUMC 14287 for CMCase(22.0 IU/ml/min)and avicellase(47.868 IU/ml/min)and A.terreus AUMC 14278 for pectinase(225.43 IU/ml/min).展开更多
文摘The present study isolated and identified 32 species of endophytic mycobiota belonging to 18 genera associated with 8 wild medicinal plants collected from El-Kharga Oasis,New Valley Governorate,Egypt.Fusarium was the most common genus followed by Alternaria and Aspergillus.Convolvulus arvensis was the plant with the highest number of endophytes over the other plant species,while Moringa oleifera reported the lowest number of endophytes.In addition,the entomopathogenic fungus Beauveria bassiana;was recorded for the first time from leaves of Portulaca oleracea.One hundred and twenty-three isolates representing 32 species were screened for their abilities to produce pectinase,carboxy methyl cellulase(CMCase)and avicellase enzymes on sucrose free-Cz supplemented,individually with 1%pectin or 1%CMC or 1%avicel as a sole carbon source,respectively.Ninety-four isolates produced pectinase while 66 isolates produced cellulases.The quantitative assays of the three enzymes for high-producers were performed in submerged fermentation using sucrose-free Cz broth.Aspergillus was the superior in the production of the three enzymes with the potent strains were A.terreus AUMC 14287 for CMCase(22.0 IU/ml/min)and avicellase(47.868 IU/ml/min)and A.terreus AUMC 14278 for pectinase(225.43 IU/ml/min).
