Identification of Microorganisms in Poultry Farms in N’djamena and the Border Areas of Hadjer-Lamis and Chari-Baguirmi Chad

Introduction: On the outskirts of Ndjamena, semi-industrial poultry farming and traditional poultry farming are practised informally on almost all poultry farms in Chad. This type of poultry farming is faced with real health problems attributable to a lack of monitoring of the vaccination schedule, inadequate compliance with biosecurity measures and poor application of the Ichikawa rule based on the 5 M’s. Objective: The aim of this article is to identify the microorganisms responsible for contamination of poultry farms in the study area. Method: The study was carried out from 28/04/2022 to 31/01/2023 on the basis of 300 samples taken from feed, drinking water, droppings and scrapings from poultry housing surfaces in the 30 farms that served as a framework for our research. Sampling was of the simple random type, and farms were selected on the basis of the farmers’ consent. The data were recorded on pre-established survey forms. Our study was cross-sectional, descriptive and prospective. Bacteria were isolated using the reference method NF EN ISO 6579 for Salmonella spp. and cultured on the specific medium eosin methylene blue (EMB) for Escherichia coli, Pseudomonas and Citrobacter freundii. Results: The following results emerged from this study: Escherichia coli (5.33%), Pseudomonas (1.33%), Citrobacter freundii (12%) and Salmonella paratyphi (21.68%). Conclusion: Of the 300 samples analysed, 121 (40.33%) were contaminated with pathogens. This high level of contamination is a health problem. The study shows that biosecurity is less satisfactory on the farms visited. Nevertheless, farms with a very satisfactory level of biosafety ensure food safety and variety for the population.

Prevalence and Risk Factors of Hepatitis B among the Population at Algamosi Locality, Gezira State, Sudan

Background: The local population in developing countries is at higher risk of contracting hepatitis B (HBV) due to some of the factors they practice which promote its establishment and distribution within their communities and may constitute a burden for them. Objective: This study aimed to determine the prevalence and risk factors associated with the hepatitis B virus in populations of the Algamosi region, Gezira State, central Sudan. Method: A cross-sectional study was conducted among 492 people, populations of the Algamosi region, Gezira state in central Sudan. A questionnaire was used to collect information on socio-demographic factors, transmission, and prevention of hepatitis B infections. As well, blood samples were taken from each participant, and serum was used for rapid tests for HBsAg. Positive samples were then tested by the ELISA method for confirmation. Data were obtained using SPSS version 21 and a P value of less than 0.05 was considered statistically significant. Results: The prevalence of hepatitis B was 16.10% and was higher in men than in women (05.7 - 1.00). People aged 31 to 45 had the highest prevalence of 27 (20,600%) for HBV infection. There was a significant association between HBV and age groups (X2 = 7.816 and P value = 0.05). This study also found that there was a significant association between viral hepatitis and knowledge about transmission and prevention (P value Conclusion: Although most people who live in developing countries are at high risk for transmission of hepatitis B, this indicates the need to implement the screening policy and integrate it with other health services and create awareness such as proper treatment and surveillance for hepatitis infection in all nations.

Factors Influencing Hepatitis C Viral Infections in the Population of Algamosi Locality, Gezira State, Central Sudan

Hepatitis C (HCV) remains a global health challenge and is transmitted via contact with contaminated blood and body fluids. This study aimed To find the factors influencing hepatitis c viral infections and to identify its prevalence among populations of the Algamosi area, Gezira state central Sudan. This cross-sectional community-based study was conducted. A total of 492 participants were randomly selected from among 4 villages and were interviewed to find out the factors that lead to transmission of infection and tested for markers of hepatitis C infection. ELISA confirmed all the positive card tests. The results showed that the individuals within the age groups > 45 and 31 - 45 years are more affected by HCV the percentage was 3.37% and 0.6% respectively, while the age groups of 15 - 30 and p < 0.05). Among the possible cause (1.4%) had a previous history of tattooing and were significantly reactive for anti-HCV (X2 = 14.588 and p value = 0.001) The study concluded that the prevalence of hepatitis C viruses was only detected in three areas. This study explored many possible causes associated with viral hepatitis, tattooing is the common cause of hepatitis C virus. Based on the finding the study recommends, the crucial intervention program to screen most of the Algamosi population who have been not screened for HCV, proper treatment for HCV, and the health education program is strongly recommended to create awareness among the general population.