Objective:To determine the antibacterial and anti-candidal activities of fatty acid methyl esters(FAME)extracted from marine microalga Nannochloropsis oculata and evaluate the inhibition activity of DNA isolated from ...Objective:To determine the antibacterial and anti-candidal activities of fatty acid methyl esters(FAME)extracted from marine microalga Nannochloropsis oculata and evaluate the inhibition activity of DNA isolated from test pathogenic microorganism.Methods:FAME was synthesized by transesterification of oil using immobilized lipase and characterized using gas chromatography-mass spectrometer.The FAME profile was determined using gas chromatography.The antimicrobial effect was tested by disc diffusion method against Gram-positive bacteria Staphylococcus aureus,Bacillus subtilis,Gram-negative bacteria Escherichia coli,Pseudomonas aeruginosa and yeast Candida albicans,at varying concentrations of 10,20 and 30μL/disc.Results:The results shown that palmitic acid(C16:0),oleic acid(C18:1)and arachidic acid(C20:0)were dominant in Nannochloropsis oculata oil.The study revealed that FAME was more active against Gram-negative than against Gram-positive and yeast.DNA inhibition activity results also confirmed that FAME had the bactericidal effect that was revealed by sheared fragments of DNA.Conclusions:The results indicated that microalgal FAME could be potentially utilized as a newer and good source of therapeutic agent in pharmaceutical industry.展开更多
基金Supported by University Grants Commission,New Delhi,India.
文摘Objective:To determine the antibacterial and anti-candidal activities of fatty acid methyl esters(FAME)extracted from marine microalga Nannochloropsis oculata and evaluate the inhibition activity of DNA isolated from test pathogenic microorganism.Methods:FAME was synthesized by transesterification of oil using immobilized lipase and characterized using gas chromatography-mass spectrometer.The FAME profile was determined using gas chromatography.The antimicrobial effect was tested by disc diffusion method against Gram-positive bacteria Staphylococcus aureus,Bacillus subtilis,Gram-negative bacteria Escherichia coli,Pseudomonas aeruginosa and yeast Candida albicans,at varying concentrations of 10,20 and 30μL/disc.Results:The results shown that palmitic acid(C16:0),oleic acid(C18:1)and arachidic acid(C20:0)were dominant in Nannochloropsis oculata oil.The study revealed that FAME was more active against Gram-negative than against Gram-positive and yeast.DNA inhibition activity results also confirmed that FAME had the bactericidal effect that was revealed by sheared fragments of DNA.Conclusions:The results indicated that microalgal FAME could be potentially utilized as a newer and good source of therapeutic agent in pharmaceutical industry.
