The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chai...The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chain reaction （PCR） was performed for further confirmation. A total of 50 blood samples were collected from poultry birds showing the malarial symptoms. The results of blood smear methods showed two samples （4%） were infected with genus Plasmodium, whereas the PCR analysis showed four （8%） positive samples. These results confirm that the PCR is more sensitive method for detecting the Plasmodium when compared with conventional methods, and the microscopy diagnosed 50% false negative results that were confirmed by PCR.展开更多
文摘The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chain reaction （PCR） was performed for further confirmation. A total of 50 blood samples were collected from poultry birds showing the malarial symptoms. The results of blood smear methods showed two samples （4%） were infected with genus Plasmodium, whereas the PCR analysis showed four （8%） positive samples. These results confirm that the PCR is more sensitive method for detecting the Plasmodium when compared with conventional methods, and the microscopy diagnosed 50% false negative results that were confirmed by PCR.
